The role of protein-protein interactions mediated by the PB1 domain of NLP transcription factors in nitrate-inducible gene expression

BMC Plant Biology
Mineko Konishi, Shuichi Yanagisawa

Abstract

NIN-LIKE PROTEIN (NLP) transcription factors are master regulators of nitrate-inducible gene expression in higher plants. NLP transcription factors contain a nitrate signal-responsive domain in the amino-terminal region, an RWP-RK-type DNA-binding domain in the middle, and a Phox and Bem1 (PB1) domain at the carboxy terminus. Although the PB1 domain of NLP transcription factors appears to mediate protein-protein interactions associated with nitrate-inducible gene expression in higher plants, its precise role in nitrate-inducible gene expression has not previously been characterized. Yeast two-hybrid assays with the PB1 domain of the Arabidopsis transcription factor NLP7 revealed NLP-NLP interactions that required the core amino acid residues (K867, D909, D911, and E913) within the PB1 domain. Consistent with previous speculation on redundant and overlapping functions between different Arabidopsis NLP transcription factors, NLP-NLP interactions were observed between a variety of combinations of different NLP transcription factors. Furthermore, a mutated form of NLP7 that harbored amino acid substitutions at K867, D909, D911, and E913 required a far higher level of expression than wild-type NLP7 to restore nitrate-responsive gene...Continue Reading

References

Jan 1, 1992·Methods in Enzymology·K R LuehrsenV Walbot
Jun 6, 2003·The Plant Journal : for Cell and Molecular Biology·Keiichiro HiratsuMasaru Ohme-Takagi
Mar 24, 2005·Journal of Molecular Evolution·Leif SchauserJens Stougaard
Aug 30, 2007·Science's STKE : Signal Transduction Knowledge Environment·Hideki SumimotoTakashi Ito
Oct 2, 2008·The Plant Journal : for Cell and Molecular Biology·Loren CastaingsAnne Krapp
Jul 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Thomas WidiezMarc Lepetit
Oct 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sandrine RuffelGloria M Coruzzi
Jan 10, 2012·The Plant Cell·Joseph G Dubrovsky, Brian G Forde
Jan 18, 2013·Plant & Cell Physiology·Naoya SawakiShuichi Yanagisawa
Mar 21, 2013·Nature Communications·Mineko Konishi, Shuichi Yanagisawa
Mar 28, 2013·DNA and Cell Biology·Xiaolong LinZuo Wang
Feb 20, 2014·Frontiers in Plant Science·Katarzyna Zientara-Rytter, Agnieszka Sirko
Jul 10, 2014·Journal of Experimental Botany·Mineko Konishi, Shuichi Yanagisawa
Oct 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Peizhu GuanNigel M Crawford
May 24, 2016·Molecular Plant·José A O'BrienRodrigo A Gutiérrez
Dec 28, 2016·Biochemical and Biophysical Research Communications·Takeo SatoShuichi Yanagisawa
Feb 17, 2017·Proceedings of the National Academy of Sciences of the United States of America·Peizhu GuanNigel M Crawford
Dec 14, 2017·Quarterly Reviews of Biophysics·Yahli Lorch, Roger D Kornberg
Jan 9, 2018·Journal of Experimental Botany·Diana E GrasRodrigo A Gutiérrez

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Citations

Jun 5, 2019·Cellular and Molecular Life Sciences : CMLS·Xiaohuan Mu, Jie Luo
Dec 4, 2019·The Plant Journal : for Cell and Molecular Biology·Min GeHan Zhao
Sep 3, 2020·Plant Biotechnology Journal·Jie WuCheng-Bin Xiang
Mar 4, 2020·Scientific Reports·Sumanth Kumar Mutte, Dolf Weijers
Sep 2, 2020·Journal of Experimental Botany·Amel MaghiaouiLiên Bach
Dec 29, 2020·Trends in Plant Science·Pierre GautratSandrine Ruffel
Mar 29, 2021·The FEBS Journal·Jeffrey R AllenLucia C Strader
May 30, 2021·Journal of Experimental Botany·Mineko KonishiShuichi Yanagisawa

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Methods Mentioned

BETA
transgenic
two-hybrid
Y2H
PCR
transfection

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