The role of reactive oxygen species in the herbicide acetochlor-induced DNA damage on Bufo raddei tadpole liver

Aquatic Toxicology
Yang LiuDejun Huang

Abstract

After exposure of Bufo raddei tadpoles to acetochlor (ACETO) for 14 days, malondialdehyde (MDA) and DNA-single strand break (DNA-SSB) in livers were analyzed. An enhanced accumulation of MDA suggests that ACETO causes oxidative stress, and the significant increase in the level of DNA-SSB indicates that ACETO induces DNA damage in a dose-dependent manner as well. On the basis of the fact that oxidative stress is caused by excessive production of reactive oxygen species (ROS), and the present results, we speculate that ACETO-induced DNA damage may be a consequence of the generation of ROS. To evaluate this hypothesis, tadpoles were treated with ROS scavenger, N-acetyl-L-cysteine (NAC) or melatonin (MEL), prior to ACETO exposure. The decrease of DNA-SSB level and the increase of total antioxidant capability (TAC) show that ACETO-caused DNA damage can be attenuated by NAC and MEL. In addition, a negative correlation was observed between the extent of DNA damage and the level of TAC in tadpole liver. In conclusion, the results suggest that ACETO-induced DNA damage is mediated by ROS.

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Citations

Oct 16, 2010·International Journal of Environmental Research and Public Health·Christopher Ddidigwu NwaniSatish Kumar Srivastava
Sep 24, 2015·Journal of Applied Toxicology : JAT·Mei YangGuonian Zhu
Oct 8, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Tamanna ZerinYong-Sik Kim
Jun 5, 2013·Environmental Toxicology and Pharmacology·Joanna BernasinskaAneta Koceva-Chyla
Oct 13, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·C D NwaniS K Srivastava
Aug 26, 2019·Environmental Science and Pollution Research International·Mohamed FathyAlaa El-Din H Sayed
May 13, 2020·Environmental Science and Pollution Research International·Yingying ZhangWenzhi Wei
Aug 21, 2021·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Wen XueWenzhi Wei
Feb 1, 2008·Journal of Agricultural and Food Chemistry·Jianfa LiHuaping Dong

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