DOI: 10.1101/506345Dec 25, 2018Paper

The role of Selenoprotein N in the differentiation of erythroid progenitors during stress erythropoiesis

BioRxiv : the Preprint Server for Biology
Robert F PaulsonK Sandeep Prabhu

Abstract

Low serum Se is independently associated with anemia in elderly population, dialysis patients, sickle-cells patients, and hypothyroidism patients. Previous work from our laboratory showed that dietary Se deficiency in mice showed mild anemia indicating activation of stress- erythropoietic mechanisms. Unlike steady state erythropoiesis that is primarily responsible for homeostasis to produce new erythrocytes at a constant rate, stress erythropoiesis predominates when the bone marrow cannot generate sufficient erythrocytes. During such a process, short-term reconstituting hematopoietic stem cells (CD34+Kit+Sca1+Linneg) migrate to the spleen leading to the proliferation and differentiation of stress-erythroid progenitors (SEPs). These cells lead to stress burst forming unit- erythroid cells (BFU-E) followed by terminal differentiation to erythrocytes. Recent studies demonstrate deficits in selenoproteins block the expansion and development of stress BFU-E with defects in terminal differentiation. Analysis of selenoprotein expression showed that selenoprotein W (SelenoW) was highly expressed in developing SEPs. CRISPR-Cas9 knockout of SelenoW blocked the proliferation of immature SEPs in murine and human stress erythropoiesis cultu...Continue Reading

Related Concepts

Anemia
Bone Marrow
Cell Differentiation Process
Cell Growth
Dialysis Procedure
Erythrocytes
Erythropoiesis
Hematopoietic Stem Cells
Hypothyroidism
Laboratory

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