The Role of Single-Cell Technology in the Study and Control of Infectious Diseases.

Cells
Weikang Nicholas LinLih Feng Cheow

Abstract

The advent of single-cell research in the recent decade has allowed biological studies at an unprecedented resolution and scale. In particular, single-cell analysis techniques such as Next-Generation Sequencing (NGS) and Fluorescence-Activated Cell Sorting (FACS) have helped show substantial links between cellular heterogeneity and infectious disease progression. The extensive characterization of genomic and phenotypic biomarkers, in addition to host-pathogen interactions at the single-cell level, has resulted in the discovery of previously unknown infection mechanisms as well as potential treatment options. In this article, we review the various single-cell technologies and their applications in the ongoing fight against infectious diseases, as well as discuss the potential opportunities for future development.

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Citations

Sep 3, 2020·Cells·Tuhin Subhra Santra, Fan-Gang Tseng
Jan 19, 2021·Journal of Electroanalytical Chemistry·Hüseyin Oğuzhan KayaSeda Nur Topkaya
May 9, 2021·European Journal of Immunology·Xiao WangFuping You
Nov 9, 2021·Molecular Metabolism·Tomás DurajAngel Ayuso-Sacido

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Methods Mentioned

BETA
single-cell sequencing
flow cytometry
imaging technology
FACS
scRNA-seq
flow cytometery
Assay
immunoprecipitation
RNA seq
Protein Sequencing

Software Mentioned

ComBat
CyTOF
BEARscc ( Bayesian ERCC Assessment of Robustness )

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