The role of site-specific N-glycosylation in secretion of soluble forms of rabies virus glycoprotein

Glycobiology
Boguslaw S WojczykS L Spitalnik

Abstract

Rabies virus glycoprotein is important in the biology and pathogenesis of neurotropic rabies virus infection. This transmembrane glycoprotein is the only viral protein on the surface of virus particles, is the viral attachment protein that facilitates virus uptake by the infected cell, and is the target of the host humoral immune response to infection. The extracellular domain of this glycoprotein has N-glycosylation sequons at Asn37, Asn247, and Asn319. Appropriate glycosylation of these sequons is important in the expression of the glycoprotein. Soluble forms of rabies virus glycoprotein were constructed by insertion of a stop codon just external to the transmembrane domain. Using site-directed mutagenesis and expression in transfected eukaryotic cells, it was possible to compare the effects of site-specific glycosylation on the cell-surface expression and secretion of transmembrane and soluble forms, respectively, of the same glycoprotein. These studies yielded the surprising finding that although any of the three sequons permitted cell surface expression of full-length rabies virus glycoprotein, only the N-glycan at Asn319 permitted secretion of soluble rabies virus glycoprotein. Despite its biological and medical importanc...Continue Reading

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Citations

Dec 1, 2009·Nature Reviews. Microbiology·Matthias J SchnellAmy Papaneri
Aug 26, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Angélique LewiesDu Plessis Lissinda Hester
Aug 16, 2016·Cellular Immunology·Braira WahidShazia Rafique
Jul 6, 2006·Virology·Mary Ellen SmithMatthias J Schnell
Jun 3, 2004·The Journal of Biological Chemistry·Alexander N R WeberNicholas J Gay
Aug 16, 2005·The Journal of General Virology·Ludmilla SissoëffChristine Tuffereau
Feb 16, 2017·The Journal of Biological Chemistry·Bin WangYong-Hui Zheng
Sep 21, 2004·The Journal of Biological Chemistry·Kunio NakatsukasaToshiya Endo
Jul 23, 2020·Cell Reports·Christine R FisherMatthias J Schnell

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