The role of sodium citrate in the dysfibrinogenaemia of liver disease

Thrombosis Research
J L FrancisV J Simmonds

Abstract

The addition of excess sodium citrate to plasma was found to inhibit fibrin polymerisation (clot opacity) from patients with cirrhosis, hepatitis and hepatoma but not from normal controls. Abnormal clot opacity in plasma from patients with liver disease could be partly or completely abolished by removal of citrate ions by dialysis against citrate-free buffer, but not by dialysis against buffer containing citrate. Similar results were observed in plasma freed of calcium ions by treatment with EGTA. Treatment of plasma with neuraminidase largely abolished the inhibitory effect of excess citrate, and the thrombin times and clot opacity of asialofibrinogen were less affected by citrate than native fibrinogen. In addition, the effects of citrate on the clotting of purified, calcium-free fibrinogen from cirrhotic patients correlated with the sialic acid content. It is concluded that binding of citrate ions to fibrinogen renders the molecule acutely more sensitive to elevations in the sialic acid content, and that a simple plasma clot opacity test in the presence of excess citrate may be a useful aid in the differential diagnosis of liver disease. These findings may also explain why defects in fibrin polymerisation observed in plasma ...Continue Reading

References

Nov 1, 1976·British Journal of Haematology·G GreenL Poller
Feb 1, 1978·The Journal of Clinical Investigation·J MartinezD Kwasniak
Jan 1, 1978·Journal of Clinical Pathology·R D BarrJ L McPhie
Feb 1, 1977·British Journal of Haematology·D A LaneR Williams
Nov 1, 1974·Thrombosis Research·A C Teger-Nilsson, H Ekelund
Jan 1, 1982·Haemostasis·J L Francis, D J Armstrong
Jun 1, 1981·Thrombosis Research·W NieuwenhuizenJ Hermans
Jun 1, 1982·Journal of Clinical Pathology·J L Francis, D J Armstrong
Jan 1, 1982·Haemostasis·J L Francis, D J Armstrong
Feb 1, 1981·Thrombosis Research·V A BelitserA A Musjalkovskaja
Jul 1, 1980·Thrombosis Research·J SoriaL Poller

Citations

Oct 1, 1985·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·O Takahashi

Related Concepts

Hereditary Factor I Deficiency Disease
Blood Coagulation
Citrates
Differential Diagnosis
Gamma-Fibrinogen
Liver Dysfunction
Sialic Acids
Thrombin Time Assay
Citric Acid, Anhydrous

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