PMID: 37935Nov 1, 1978

The role of solvents in the stabilization of helical structure: the low pH ribo A8 and A10 double helices in mixed solvents

Biophysical Chemistry
K J BreslauerJ E McCarthy

Abstract

The order-disorder transitions of the double helices formed by the ribo-oligoadenylic acids rA8 and rA10 at pH 4.2 have been investigated in a series of organic/aqueous mixed solvents. Melting temperature data, Tm, derived from the uv melting curves were used to define the stability of the double helices in the different mixed solvent systems. It was found that the extent of helix destabilization depended in a predictable fashion on both the quantity and the nature of the added organic solvents. For the C1 through C4 aliphatic alcohols, the longer, less branched alcohols proved to be more effective destabilizers of the helical structure. Significantly, the amides proved to be more powerful destabilizers than the alcohols. Analysis of the melting curves provided the Van't Hoff enthalpy change for each transition. The data are interpreted in terms of the role of solvent in the stabilization of ribonucleic acid structure.

References

Jan 2, 1995·Biochimica Et Biophysica Acta·S Bone, C A Small
Apr 10, 1996·Biochimica Et Biophysica Acta·S BoneC E Hodgson
Jun 19, 1998·Biochimica Et Biophysica Acta·R S Lee, S Bone
Oct 13, 1998·RNA·Andrew L FeigO C Uhlenbeck
Oct 15, 1996·Nucleic Acids Research·J DuhamelP Lu
Mar 17, 1981·Biochemistry·D D Albergo, D H Turner
Apr 9, 1985·Biochemistry·D R Hickey, D H Turner

Citations

Jul 1, 1961·Archives of Biochemistry and Biophysics·T T HERSKOVITSE P GEIDUSCHEK

Related Concepts

Thermodynamics
Macromolecular Compounds
RNA Conformation
DNA Conformation
Solvents
Hydrogen-Ion Concentration
RNA

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