PMID: 8593124Oct 1, 1995Paper

The role of stable isotopes in the investigation of plasma lipoprotein metabolism

Baillière's Clinical Endocrinology and Metabolism
C J Packard

Abstract

The last 5 years have seen promising beginnings of the application of stable-isotope-based methods to the study of lipoprotein metabolism. Many aspects of plasma lipid transport make it an attractive system for investigating by this means. While early efforts borrowed from standard techniques of generating and interpreting kinetic parameters (FSRs), the shortcomings of these procedures as applied to lipoproteins are now appreciated. Lipoprotein heterogeneity requires that multicompartmental analysis and relatively long-term studies be employed if the information obtained is going to be a useful adjunct to that produced by radio-iodinated lipoprotein tracers. Both approaches-stable-isotope-labeled endogenous tracer and ex vivo radio-iodinated lipoprotein experiments--must be considered complementary. The first provides direct information on lipoprotein synthesis pathways while the latter is superior at following interconversions and catabolic events. Excellent agreement has been demonstrated where the methods have been used simultaneously to estimate the same kinetic parameter. Many of the questions that have arisen from decades of radioactive tracer studies relate to the nature and rate of lipoprotein synthesis, e.g. what kinds...Continue Reading

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Citations

Sep 18, 1998·Diabetes Research and Clinical Practice·G Steiner
Jun 6, 2007·Journal of Lipid Research·Iqbal A R Al-ShayjiMuriel J Caslake
Aug 1, 1997·Clinical and Experimental Pharmacology & Physiology·G F WattsK D Croft
Jul 16, 2010·Expert Opinion on Pharmacotherapy·Dick C Chan, Gerald F Watts

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