The Role of Stress Fibers in the Shape Determination Mechanism of Fish Keratocytes

Biophysical Journal
Takako NakataYoshiaki Iwadate

Abstract

Crawling cells have characteristic shapes that are a function of their cell types. How their different shapes are determined is an interesting question. Fish epithelial keratocytes are an ideal material for investigating cell shape determination, because they maintain a nearly constant fan shape during their crawling locomotion. We compared the shape and related molecular mechanisms in keratocytes from different fish species to elucidate the key mechanisms that determine cell shape. Wide keratocytes from cichlids applied large traction forces at the rear due to large focal adhesions, and showed a spatially loose gradient associated with actin retrograde flow rate, whereas round keratocytes from black tetra applied low traction forces at the rear small focal adhesions and showed a spatially steep gradient of actin retrograde flow rate. Laser ablation of stress fibers (contractile fibers connected to rear focal adhesions) in wide keratocytes from cichlids increased the actin retrograde flow rate and led to slowed leading-edge extension near the ablated region. Thus, stress fibers might play an important role in the mechanism of maintaining cell shape by regulating the actin retrograde flow rate.

References

Nov 1, 1988·Neuron·T Mitchison, M Kirschner
Mar 11, 1993·Nature·J LeeK Jacobson
Feb 9, 1996·Cell·D A Lauffenburger, A F Horwitz
Mar 30, 1999·Biophysical Journal·M Dembo, Y L Wang
Nov 17, 1999·Molecular Biology of the Cell·K BurtonD L Taylor
Jan 12, 2000·The Journal of Cell Biology·D Raucher, M P Sheetz
Jun 27, 2000·Biophysical Journal·C M LoY L Wang
Dec 12, 2001·Molecular Biology of the Cell·S MunevarM Dembo
Nov 26, 2002·The Journal of Cell Biology·Catherine G GalbraithMichael P Sheetz
Dec 6, 2003·Science·Anne J RidleyAlan Rick Horwitz
Mar 24, 2004·Current Opinion in Cell Biology·Carole A Parent
Jul 20, 2007·Molecular Biology of the Cell·Sébastien SchaubAlexander B Verkhovsky
Apr 5, 2008·Journal of Cell Science·Yoshiaki Iwadate, Shigehiko Yumura
May 24, 2008·Nature·Kinneret KerenJulie A Theriot
Jun 4, 2008·The Journal of Cell Biology·Tadayuki ShimadaNaoyuki Inagaki
Dec 17, 2008·Science·Clarence E Chan, David J Odde
Aug 12, 2009·Journal of Biomechanics·Kennedy Omondi OkeyoMasaki Hojo
Sep 1, 2009·Trends in Cell Biology·Grégory GiannoneOlivier Thoumine
Nov 13, 2009·Current Biology : CB·Alex Mogilner, Kinneret Keren
Jan 27, 2010·The Journal of Cell Biology·Maxime F FournierAlexander B Verkhovsky
May 12, 2011·PLoS Biology·Erin L BarnhartJulie A Theriot
Nov 29, 2011·Cell·Peter Friedl, Stephanie Alexander
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Qingzong TsengManuel Théry
Jun 13, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Hiromi Miyoshi, Taiji Adachi
Jun 22, 2013·The Journal of Experimental Biology·Harunobu TsugiyamaYoshiaki Iwadate
Jul 9, 2013·Current Biology : CB·Arnon D LieberKinneret Keren
Oct 16, 2013·Cytoskeleton·Thomas FuhsJosef A Kaes
Apr 12, 2015·Biophysical Journal·Arnon D LieberKinneret Keren

❮ Previous
Next ❯

Citations

Apr 29, 2016·Cell Adhesion & Migration·Chika Okimura, Yoshiaki Iwadate
Jun 17, 2018·Physical Review. E·Chika OkimuraYoshiaki Iwadate
Jul 19, 2018·Scientific Reports·Chika OkimuraYoshiaki Iwadate

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.