PMID: 11902330Mar 21, 2002Paper

The role of surface ig binding in the activation of human B cells by phosphorothioate oligodeoxynucleotides

Scandinavian Journal of Immunology
H LiangP E Lipsky

Abstract

Phosphorothioate oligodeoxynucleotides (sODNs) can induce T-cell-independent polyclonal activation of human B cells by a mechanism that depends on both sequence and back-bone structure. Because matrix-bound as well as soluble sODNs are mitogenic, this stimulation may result from the engagement of surface receptor(s). In order to investigate whether surface immunoglobin (Ig) could be a receptor for sODNs, the interaction of sODNs-fluorescein isothiocyanate (FITC) with Ig-coated beads was examined. sODNs specifically bound to human IgM and IgG. Moreover, binding of sODN to human B cells induced temperature-dependent capping of bound receptors and colocalization of FITC-sODN and IgM into aggregated caps on the surface of human B cells. A role of surface Ig was furthermore shown by observations that antibody-mediated capping of B-cell surface IgM or IgD inhibited subsequent binding of sODNs and that the capacity of sODN to stimulate human B cells was blocked by excess IgM or IgG, by nonstimulatory antibodies to sIgM, as well as by a variety of negatively charged molecules. Together, these results indicate that sODNs engage surface Ig by charge-charge interactions that lead to activation of human B cells.

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Citations

Jan 25, 2005·Oligonucleotides·Liang-Hao Guo, Hermann J Schluesener
Dec 30, 2014·Cellular and Molecular Life Sciences : CMLS·Mariann KremlitzkaAnna Erdei
Jul 5, 2006·International Reviews of Immunology·Jörg Vollmer
Feb 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kunio SanoGen Tamura
Aug 12, 2009·Analytical Chemistry·Binh T T NguyenChee-Seng Toh

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