The Role of Telomerase in Radiation-Induced Genomic Instability

Radiation Research
Otilia NutaFirouz Darroudi

Abstract

Findings from previous studies have suggested that the telomerase system is involved in radiation-induced genomic instability. In this study, we investigated the involvement of telomerase in the development and processing of chromosomal damage at different cell cycle stages after irradiation of human fibroblasts. Several response criteria were investigated, including cell survival, chromosomal damage (using the micronucleus assay), G2-induced chromatid aberrations (using the conventional G2 assay as well as a chemically-induced premature chromosome condensation assay) and DNA double-strand breaks (DSBs; using γ-H2AX, 53BP1 and Rad51) in an isogenic pair of cell lines: BJ human foreskin fibroblasts and BJ1-hTERT, a telomerase-immortalized BJ cell line. To distinguish among G1, S and G2 phase, cells were co-immunostained for CENP-F and cyclin A, which are tightly regulated proteins in the cell cycle. After X-ray irradiation at doses in the range of 0.1-6 Gy, the results showed that for cell survival and micronuclei induction, where the overall effect is dominated by the cells in G1 and S phase, no difference was observed between the two cell types; in contrast, when radiation sensitivity at the G2 stage of the cell cycle was anal...Continue Reading

References

May 31, 1990·Nature·C B HarleyC W Greider
Feb 1, 1985·Mutation Research·M Fenech, A A Morley
Sep 1, 1995·Science·J FengJ Yu
Jan 1, 1995·Cytogenetics and Cell Genetics·T K PanditaC R Geard
Sep 1, 1995·Experimental Cell Research·R C AllsoppC B Harley
Nov 1, 1994·International Journal of Radiation Biology·L SabatierB Dutrillaux
May 10, 2000·Current Opinion in Cell Biology·K Collins
Sep 7, 2001·Advances in Space Research : the Official Journal of the Committee on Space Research (COSPAR)·C L LimoliW F Morgan
Nov 2, 2001·Nature·T ReyaI L Weissman
Jan 16, 2002·Current Opinion in Genetics & Development·Dara L AisnerJerry W Shay
Feb 14, 2002·Differentiation; Research in Biological Diversity·Nicholas R ForsythJerry W Shay
May 22, 2003·Cancer Letters·Chantal DesmazeLaure Sabatier
May 1, 1956·The Journal of Experimental Medicine·T T PUCK, P I MARCUS
Nov 11, 2003·Nature·Fabrizio d'Adda di FagagnaStephen P Jackson
Apr 28, 2004·Cell·Madalena TarsounasStephen C West
Jun 8, 2004·Molecular Biology of the Cell·Ying ZouWoodring E Wright
Oct 23, 2004·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·John P Murnane, Laure Sabatier
Dec 22, 2004·Mechanisms of Ageing and Development·T von ZglinickiS P Jackson
May 26, 2005·Mutation Research·Lieneke R van VeelenRoland Kanaar
Jun 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Kenkichi MasutomiWilliam C Hahn
Nov 26, 2005·Molecular Cell·Ramiro E VerdunJan Karlseder
Jan 15, 2008·Radiation and Environmental Biophysics·Otilia Nuta, Firouz Darroudi
Sep 18, 2008·British Journal of Cancer·U TaboriC Hawkins
Feb 25, 2011·Nature Reviews. Cancer·Paula Martínez, María A Blasco
Mar 20, 2012·Nature Cell Biology·Marzia FumagalliFabrizio d'Adda di Fagagna
Sep 14, 2012·Frontiers in Oncology·Daniel E GomezDaniel F Alonso
Oct 13, 2012·Frontiers in Oncology·Keiko MurakiJohn P Murnane
Jul 10, 2014·BMJ : British Medical Journal·Philip C HaycockPeter Willeit
Feb 14, 2016·Proceedings of the National Academy of Sciences of the United States of America·Bjoern SchwerFrederick W Alt
Jun 25, 2016·Nature Reviews. Cancer·Greg M Arndt, Karen L MacKenzie
May 13, 2018·Genes·Tiago Bordeira GasparJoão Vinagre
Nov 23, 2018·Genes & Development·Héloïse CoutelierMaria Teresa Teixeira

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