PMID: 6159586Sep 25, 1980Paper

The role of the basic N-terminal region of protein L18 in 5S RNA-23S RNA complex formation

Nucleic Acids Research
V Newberry, R A Garrett

Abstract

Of the three proteins, L5, L18 and L25, which bind to 5S RNA, the former two effect the interaction of 5S RNA with 23S RNA. We have used trypsin as a probe to investigate the roles of the proteins in this RNA-RNA assembly, with the following results: (1) In complexes with 5S RNA, the highly basic N-terminal region of L18 is accessible to trypsin. This accessibility is unaffected by L25. However, its presence is essential for stimulating L5 binding. (2) In 5S RNA-protein-23S RNA complexes proteins L5 and L18 are both strongly resistant to proteolysis. (3) No 5S RNA-23S RNA complex formation occurs in the presence of L5 and the C-terminal L18 fragment. Two possible models for the mechanism of RNA-RNA assembly are proposed.

References

Jul 1, 1977·Nucleic Acids Research·D G BearR A Garrett
Aug 7, 1975·Nature·G E Fox, C R Woese
Oct 26, 1973·Biochimica Et Biophysica Acta·R S Yu, H G Wittmann
Nov 11, 1971·European Journal of Biochemistry·I HindennachH G Wittmann

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Citations

Apr 20, 2002·The Biochemical Journal·Esmeralda A WoestenenkHelena Berglund
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R Röhl, K H Nierhaus
Feb 17, 2009·Biochemistry. Biokhimii︠a︡·G M GongadzeM B Garber
Nov 14, 1998·Journal of Molecular Biology·P OstergaardR A Garrett
Mar 17, 2018·Biochemistry. Biokhimii︠a︡·A D Nikulin
Mar 26, 1991·Biochimica Et Biophysica Acta·N KenmochiT Tanaka

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