PMID: 6989597Feb 1, 1980Paper

The role of the codon and the initiation factor IF-2 in the selection of N-blocked aminoacyl-tRNA for initiation

European Journal of Biochemistry
K Van Der LakenP Van Knippenberg

Abstract

Poly(uridylic acid) [poly(U)] and poly(xanthidylic acid) [poly(X)] strongly stimulate the IF-2-dependent binding of fMET-tRNA to 30-S ribosomal subunits from Escherichia coli [Van der Laken et al. (1979) FEBS Lett. 100, 230-234]. The N-formylmethionine moiety is incorporated into poly(phenylalanine) upon subsequent addition of other components required for protein synthesis when poly(U) is used as template. This paper shows that N-acetylated Phe-tRNAPhe (AcPhe-tRNA), but not Phe-tRNAPhe or tRNAPhe, competes with fMET-tRNA for binding to poly(U)-programmed 30-S ribosomal subunits. The two species of N-blocked aminoacyl-tRNA are bound to poly(U)-programmed and poly(X)-programmed 30-S subunits in a ratio that is linearly dependent on the ratio of the two species added. With poly(U) as template there is no apparent preference for either fMET-tRNA or AcPhe-tRNA, whereas with poly(X) there is a 2-3-fold preference for fMET-tRNA. The initiation factor IF-2, which is strictly required for the binding of N-blocked aminoacyl-tRNAs, has a higher affinity for fMET-tRNA than for AcPhe-tRNA. It is concluded that (a) interaction of the 30-S ribosomal subunit with poly(U) or poly(X) leads to IF-2-dependent binding of N-blocked aminoacyl-tRNA; ...Continue Reading

References

Aug 1, 1977·Archives of Biochemistry and Biophysics·J W HersheyJ L Fakunding
Jul 1, 1977·European Journal of Biochemistry·G A van der HofstadH O Voorma
Jun 25, 1975·Journal of Molecular Biology·J G FilesJ H Miller
Feb 26, 1968·Biochimica Et Biophysica Acta·N J VerhoefL Bosch
Oct 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·M B HilleA J Wahba
Feb 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·J Lucas-Lenard, A L Haenni
Feb 28, 1969·Journal of Molecular Biology·M Grunberg-ManagoJ Dondon
Jan 18, 1966·Biochimica Et Biophysica Acta·A L Haenni, F Chapeville
Jun 1, 1966·Journal of Molecular Biology·B F Clark, K A Marcker
Apr 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·J Lucas-Lenard, F Lipmann
Feb 1, 1971·European Journal of Biochemistry·H O VoormaT J den Hertog

Citations

Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·L GoldR Saunders
May 11, 1982·Nucleic Acids Research·G D StormoL Gold
Jun 7, 2016·Molecular Cell·Angela M ChevertonSophie Helaine
Jan 30, 2020·PLoS Biology·Daria S VinogradovaPohl Milón

Related Concepts

Sense Codon
Alkalescens-Dispar Group
Peptide Initiation Factors
Polyribonucleotides
Ribosomes
Poly(A) Tail
RNA, Transfer, Amino Acyl
Structure-Activity Relationship
Eukaryotic Initiation Factor-2, gamma Subunit

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