PMID: 12774890May 31, 2003Paper

The role of the cytoskeleton in mechanotransduction in human osteoblast-like cells

Human & Experimental Toxicology
Nahum Rosenberg

Abstract

The regulation of osteoblast proliferation is a key factor in maintaining bone mass. The enhancement of this process can be achieved by stimulating the proliferation of these cells. Mechanical stimulation is one of the important enhancing factors, but the exact cellular mechanisms of mechanical stimulation, i.e., mechanotransduction, are unknown. In order to investigate the role of the cytoskeleton components in mechanotransduction for cell proliferation, I compared the total DNA content in cultured replicates of osteoblast-like cells derived from three human donors following their exposure to enhancing mechanical stimulation, with and without added specific microtubular and microfilament polymerization blockers (Colchicin and Cytochalasin D, respectively). The results revealed the essential and unique role of the microtubular component of the cytoskeleton in mechanotransduction for proliferation by showing that Colchicin blocked the expected increase in the DNA content after mechanical stimulation of the cultured replicates without altering the total DNA content in replicates at static conditions. Conversely, a specific blockage of the microfilament polymerization presented uniform cytotoxic effect in both static and biomechan...Continue Reading

References

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Citations

Mar 5, 2009·Tissue Engineering. Part C, Methods·Deborah PrèMaria Gabriella Cusella De Angelis
Sep 9, 2004·Science's STKE : Signal Transduction Knowledge Environment·Millie Hughes-Fulford
Jan 20, 2005·Biomedizinische Technik. Biomedical Engineering·U BoudriotJ Brandt
Jun 9, 2014·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Wei ZhangDeyu Chen
Apr 23, 2015·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Werner E G MüllerXiaohong Wang
Apr 19, 2006·Journal of Cellular Biochemistry·Millie Hughes-FulfordUta Jütting
Jan 1, 2012·Bone & Joint Research·N Rosenberg, O Rosenberg
Apr 18, 2018·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Shaun N RobertsonStuart Reid
Aug 15, 2006·Biochemical and Biophysical Research Communications·Jun LiuYangxi Chen
Jun 7, 2011·Reproductive Biomedicine Online·Vladimir IsachenkoEvgenia Isachenko

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