The Role of the Exo-Xis Region in Oxidative Stress-Mediated Induction of Shiga Toxin-Converting Prophages

Oxidative Medicine and Cellular Longevity
Katarzyna LicznerskaG Wegrzyn

Abstract

Previous studies indicated that these genetic elements could be involved in the regulation of lysogenization and prophage induction processes. The effects were dramatic in Shiga toxin-converting phage Φ24(B) after treatment with oxidative stress-inducing agent, hydrogen peroxide, while they were less pronounced in bacteriophage λ and in both phages irradiated with UV. The hydrogen peroxide-caused prophage induction was found to be RecA-dependent. Importantly, in hydrogen peroxide-treated E. coli cells lysogenic for either λ or Φ24(B), deletion of the exo-xis region resulted in a significant decrease in the levels of expression of the S.O.S. regulon genes. Moreover, under these conditions, a dramatic decrease in the levels of expression of phage genes crucial for lytic development (particularly xis, exo, N, cro, O, Q, and R) could be observed in Φ24(B)-, but not in λ-bearing cells. We conclude that genes located in the exo-xis region are necessary for efficient expression of both host S.O.S regulon in lysogenic bacteria and regulatory genes of Shiga toxin-converting bacteriophage Φ24(B).

References

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Nov 21, 2002·Journal of Molecular Biology·Kirill SergueevStuart Austin
Aug 13, 2005·Progress in Nucleic Acid Research and Molecular Biology·Grzegorz Wegrzyn, Alicja Wegrzyn
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Jan 15, 2010·FEMS Immunology and Medical Microbiology·Joanna M LośGrzegorz Wegrzyn
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Feb 4, 2012·BMC Medicine·Dirk WerberKlaus Stark
Mar 17, 2012·Advances in Virus Research·Grzegorz WęgrzynAlicja Węgrzyn
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Nov 30, 2013·Nucleic Acids Research·Robert D FinnMarco Punta

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Citations

Mar 15, 2018·Journal of Enzyme Inhibition and Medicinal Chemistry·Sylwia BlochGrzegorz Węgrzyn
Jan 24, 2020·Virus Genes·Aleksandra DydeckaBożena Nejman-Faleńczyk
Oct 14, 2018·Viruses·Aleksandra DydeckaAlicja Węgrzyn
Nov 10, 2020·Frontiers in Microbiology·Mohammed Radhi MohaisenHeather Elizabeth Allison

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Methods Mentioned

BETA
PCR
electrophoresis
Assay
reverse transcription-PCR

Software Mentioned

ClustalW
Primer3web
ImageJ

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