The role of the K-channel and the active-site tyrosine in the catalytic mechanism of cytochrome c oxidase

Biochimica Et Biophysica Acta
Vivek Sharma, Mårten Wikström

Abstract

The active site of cytochrome c oxidase (CcO) comprises an oxygen-binding heme, a nearby copper ion (CuB), and a tyrosine residue that is covalently linked to one of the histidine ligands of CuB. Two proton-conducting pathways are observed in CcO, namely the D- and the K-channels, which are used to transfer protons either to the active site of oxygen reduction (substrate protons) or for pumping. Proton transfer through the D-channel is very fast, and its role in efficient transfer of both substrate and pumped protons is well established. However, it has not been fully clear why a separate K-channel is required, apparently for the supply of substrate protons only. In this work, we have analysed the available experimental and computational data, based on which we provide new perspectives on the role of the K-channel. Our analysis suggests that proton transfer in the K-channel may be gated by the protonation state of the active-site tyrosine (Tyr244) and that the neutral radical form of this residue has a more general role in the CcO mechanism than thought previously. This article is part of a Special Issue entitled 'EBEC 2016: 19th European Bioenergetics Conference, Riva del Garda, Italy, July 2-6, 2016', edited by Prof. Paolo Be...Continue Reading

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Citations

May 4, 2016·Biochimica Et Biophysica Acta·Paolo Bernardi
Nov 15, 2017·Proceedings of the National Academy of Sciences of the United States of America·Vivek SharmaPeter R Rich
May 26, 2017·Proceedings of the National Academy of Sciences of the United States of America·Ruibin LiangGregory A Voth
Aug 27, 2017·Biochemical Society Transactions·Jian LiuShelagh Ferguson-Miller
Oct 13, 2018·Chemical Science·Shreyas Supekar, Ville R I Kaila
Nov 25, 2017·FEBS Letters·Camilla Rydström Lundin, Peter Brzezinski
Jun 18, 2017·Biochemical Society Transactions·Peter R Rich
Aug 11, 2018·Nature Communications·Markus L Björck, Peter Brzezinski
May 23, 2019·International Journal of Molecular Medicine·Ru-Zhou ZhaoZhi-Bin Yu
Feb 18, 2020·Journal of Inorganic Biochemistry·Margareta R A Blomberg
Jun 30, 2021·Chemical Reviews·Peter BrzezinskiPia Ädelroth
May 31, 2019·Journal of the American Chemical Society·Andrew W SchaeferEdward I Solomon
Jan 20, 2018·Chemical Reviews·Mårten WikströmVivek Sharma
Dec 30, 2017·Chemical Reviews·Xiongyi Huang, John T Groves
Aug 24, 2021·Journal of the American Chemical Society·JoAnne Stubbe, Daniel G Nocera
Jul 18, 2018·The Journal of Physical Chemistry. B·Ardavan Farahvash, Alexei Stuchebrukhov
Oct 30, 2018·Chemical Reviews·Suzanne M AdamKenneth D Karlin

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