The role of the lectin pathway of the complement system in SARS-CoV-2 lung injury.

Translational Research : the Journal of Laboratory and Clinical Medicine
Mineia Alessandra Scaranello MalaquiasLucia de Noronha

Abstract

Although some evidence showed the activation of complement systems in COVID-19 patients, proinflammatory status and lectin pathway remain unclear. Thus, the present study aimed to demonstrate the role of MBL and ficolin-3 in the complement system activation and compared to pandemic Influenza A virus H1N1 subtype infection (H1N1pdm09) and control patients. A total of 27 lungs formalin-fixed paraffin-embedded samples (10 from H1N1 group, 6 from the COVID-19 group, and 11 from the control group) were analyzed by immunohistochemistry using anti-IL-6, TNF-alfa, CD163, MBL e FCN3 antibodies. Genotyping of target polymorphisms in the MBL2 gene was performed by real-time PCR. Proinflammatory cytokines such as IL-6 and TNF-alpha presented higher tissue expression in the COVID-19 group compared to H1N1 and control groups. The same results were observed for ICAM-1 tissue expression. Increased expression of the FCN3 was observed in the COVID-19 group and H1N1 group compared to the control group. The MBL tissue expression was higher in the COVID-19 group compared to H1N1 and control groups. The genotypes AA for rs180040 (G/A), GG for rs1800451 (G/A) and CC for rs5030737 (T/C) showed a higher prevalence in the COVID-19 group. The intense act...Continue Reading

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Citations

Mar 9, 2021·Open Medicine·Namarta KaliaManpreet Kaur
Apr 20, 2021·Frontiers in Immunology·Marina Luise Viola AzevedoLucia de Noronha
Aug 27, 2021·Genetics and Molecular Biology·Vitor R C AguiarMaria Luiza Petzl-Erler
Sep 1, 2021·ELife·Esteban Dodero-RojasPaul Charles Whitford
Nov 16, 2021·Frontiers in Immunology·Panteleimon CharitosMichael Osthoff

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Methods Mentioned

BETA
glycosylation
biopsy
biopsies
PCR
genotyping

Software Mentioned

Image
The
R Project for Statistical Computing
Pro Plus

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