The role of the miR-31/FIH1 pathway in TGF-β-induced liver fibrosis

Clinical Science
Jiangfeng HuLiang Zhu

Abstract

The miRNAs are small, non-coding RNAs that regulate various biological processes, including liver fibrosis. Hepatic stellate cells (HSCs) play a central role in the pathogenesis of liver fibrosis. By microarray profiling and real-time PCR, we noted that miR-31 expression in HSCs from rats, mice and humans was significantly increased during HSC activation in culture. Overall, miR-31 expression levels were unchanged in the whole-liver RNA extracts from fibrotic rat and human samples. Nevertheless, we found that miR-31 was particularly up-regulated in HSCs but not in hepatocytes during fibrogenesis. Thus, we hypothesized that miR-31 may mediate liver fibrosis. In the present study, we found that inhibition of miR-31 expression significantly inhibited HSC activation, whereas its over-expression obviously promoted HSC activation. Moreover, over-expression of miR-31 promoted HSC migration by enhancing matrix metalloproteinase (MMP)-2 expression whereas inhibition of miR-31 has an opposite effect. The biological function of miR-31 during HSC activation might be through targeting FIH1, a suppressor of hypoxia-inducible factor (HIF-1), because a knockdown of FIH1 by shRNA could mimic the effects of miR-31. In addition, primary rat HSCs ...Continue Reading

References

Feb 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S L FriedmanG Yamasaki
Jul 26, 1996·The Journal of Biological Chemistry·B H JiangG L Semenza
Jun 1, 1997·Journal of Gastroenterology·S L Friedman
Jun 30, 2001·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·B SchnablD A Brenner
Jun 24, 2004·Nature Reviews. Genetics·Lin He, Gregory J Hannon
Sep 17, 2004·Nature·Victor Ambros
Feb 4, 2005·The Journal of Clinical Investigation·Ramón Bataller, David A Brenner
Mar 25, 2006·Nature Reviews. Cancer·Aurora Esquela-Kerscher, Frank J Slack
Mar 28, 2006·Journal of Cellular and Molecular Medicine·A M Gressner, R Weiskirchen
Nov 7, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Senthil K VenugopalMark A Zern
May 22, 2010·Journal of the American Society of Nephrology : JASN·Arthur C K ChungHui Y Lan
Aug 20, 2011·Journal of the American Society of Nephrology : JASN·Xiang ZhongHui Y Lan
Apr 17, 2012·Gastroenterology·Xin Wei WangHenrik Orum
Jun 5, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shanzhong YangGang Liu
Aug 2, 2012·Fibrogenesis & Tissue Repair·Masashi IizukaNorifumi Kawada
Mar 29, 2013·The New England Journal of Medicine·Harry L A JanssenMichael R Hodges
May 22, 2013·Nature Reviews. Gastroenterology & Hepatology·Gyongyi Szabo, Shashi Bala
Jan 11, 2014·Scandinavian Journal of Gastroenterology·Atsushi MasamuneTooru Shimosegawa

❮ Previous
Next ❯

Citations

Mar 22, 2016·Journal of Clinical Medicine·Mio Kitano, P Mark Bloomston
Aug 19, 2015·Frontiers in Physiology·Joeri LambrechtLeo A van Grunsven
Dec 7, 2017·International Journal of Molecular Sciences·Walter M HodgesMark W Hamrick
Nov 14, 2019·Cells·Bedair DewidarAnd Nadja Meindl-Beinker
Dec 13, 2017·Oncotarget·Jiangfeng HuHengjun Gao
Sep 20, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yueqin ZhuYan Huang
Dec 28, 2016·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Jing-Jing YangJun Li
Jul 31, 2019·International Journal of Molecular Sciences·Lin ChenXiaofeng Zhou
Jan 4, 2019·Molecular Diagnosis & Therapy·Lechun LyuXiang Li
Oct 2, 2020·International Journal of Molecular Sciences·Tomasz KmiołekAgnieszka Paradowska-Gorycka
Jun 24, 2021·PLoS Neglected Tropical Diseases·Xiaolei SunYinong Duan
May 2, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Longqiu YangXuejie Fei

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.