The role of the nitrate respiration element of Thermus thermophilus in the control and activity of the denitrification apparatus

Environmental Microbiology
Felipe CavaJosé Berenguer

Abstract

The nitrate conjugative element (NCE) encodes the ability to respire nitrate in the facultative Thermus thermophilus NAR1 strain. This process is carried out by two heterotetrameric enzymes that catalyse the oxidation of NADH (Nrc) and the reduction of nitrate (Nar), whose expression is activated by the NCE-encoded transcription factors DnrS and DnrT. We report the presence of NCE in other facultative strains of T. thermophilus and analyse its role in subsequent steps of the denitrification pathway. We encountered that nrc mutants of denitrifying strains show a decrease in anaerobic growth rates not only with nitrate, but also with nitrite, NO and N(2)O, which is concomitant to their lower NADH oxidation activities in vitro. We show that nitrate, nitrite and NO are activating signals for transcription of nrc in these strains. Finally, we demonstrate that DnrS and DnrT are required for anaerobic growth not only with nitrate, but also with nitrite, NO and N(2)O. These data allow us to conclude that: (i) Nrc constitutes the main electron donor for the four reductases of the denitrification pathway, and (ii) the NCE controls the expression of the whole denitrification pathway and the repression of the aerobic respiration through th...Continue Reading

References

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Citations

Jan 22, 2009·Extremophiles : Life Under Extreme Conditions·Felipe CavaJosé Berenguer
Jul 17, 2013·Proceedings of the National Academy of Sciences of the United States of America·Lici A Schurig-BriccioRobert B Gennis
Dec 21, 2010·Applied and Environmental Microbiology·Laura AlvarezJosé Berenguer
Feb 10, 2009·Applied and Environmental Microbiology·Naomi L WardCheryl R Kuske
Oct 22, 2013·Applied and Environmental Microbiology·Laura AlvarezJosé Berenguer
Jan 27, 2011·Biochemical Society Transactions·Carlos BricioJosé Berenguer
Feb 5, 2013·Biochimica Et Biophysica Acta·Stéphane GrimaldiAxel Magalon
Jan 21, 2017·Microbial Ecology·Arshia AminWen-Jun Li
Jan 21, 2014·Journal of Bacteriology·Laura AlvarezJosé Berenguer
Jun 8, 2014·Applied and Environmental Microbiology·C BricioJ Berenguer
Sep 7, 2018·Environmental Microbiology·Zahra ChahlafiJosé Berenguer
Aug 22, 2020·Nucleic Acids Research·Daria ArtamonovaKonstantin Severinov
Feb 28, 2019·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Laura AlvarezJosé Berenguer

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