The Role of the RhoA/ROCK Signaling Pathway in Mechanical Strain-Induced Scleral Myofibroblast Differentiation

Investigative Ophthalmology & Visual Science
Ying YuanBilian Ke

Abstract

Biomechanical properties changes and α-smooth muscle actin (α-SMA) overexpression are involved in myopia scleral remodeling. However, interactions between altered tissue biomechanics and cellular signaling that sustain scleral remodeling have not been well defined. We determine the mechanisms of mechanotransduction in the regulation of α-SMA expression during myopia scleral remodeling. Guinea pigs were used to establish a form-deprivation myopia (FDM) model. Protein profiles in myopic sclera were examined using tandem mass spectrometry. Ras homolog gene family member A (RhoA) and α-SMA expressions were confirmed using quantitative (q) RT-PCR and Western blotting. Scleral fibroblasts were cultured and subjected to 4% cyclic strain. Levels of RhoA, rho-associated protein kinase-2 (ROCK2), myocardin-related transcription factor-A (MRTF-A), serum response factor (SRF), and α-SMA were determined by qRT-PCR and Western blotting in groups with or without the RhoA siRNA or ROCK inhibitor Y27632. MRTF-A and α-SMA were evaluated by confocal immunofluorescent microscopy and myofibroblasts were enumerated using flow cytometry. mRNA and protein levels of RhoA and α-SMA were significantly increased in the FDM eyes after 4 weeks of form-depri...Continue Reading

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Feb 16, 2019·Omics : a Journal of Integrative Biology·Varshasnata MohantyKrishna R Murthy
Jan 28, 2021·Investigative Ophthalmology & Visual Science·Julia SzetoIan Pitha
Mar 2, 2021·Molecular Therapy : the Journal of the American Society of Gene Therapy·Dan LiYi Lu
Apr 29, 2021·American Journal of Physiology. Lung Cellular and Molecular Physiology·Qin Yang, Wei Shi
Nov 18, 2021·Investigative Ophthalmology & Visual Science·Peiyuan WangUNKNOWN GSHM study group
Dec 31, 2021·Investigative Ophthalmology & Visual Science·Xuan ZhouXiangtian Zhou

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