The role of the sarcolemmal Ca(2+)-ATPase in the pH transients associated with contraction in rat smooth muscle

The Journal of Physiology
E K NaderaliS Wray

Abstract

1. We have investigated the origin of the intracellular acid pH transients that accompany myometrial contraction. Intra- and extracellular pH were measured with SNARF and intracellular Ca2+ concentration ([Ca2+]i) with indo-1. 2. An intracellular acidification accompanied spontaneous contractions and those elicited by KCl depolarization or the addition of the agonists carbachol or prostaglandin F2 alpha. The size of the acidification increased with the magnitude of the contraction. 3. The intracellular acidification was accompanied by an extracellular alkalinization, showing that it results from proton movement across the surface membrane. Furthermore, it was decreased either by addition of Cd2+ (20 nM, an inhibitor of the sarcolemmal Ca(2+)-ATPase) or by elevating [Ca2+]o. 4. Extracellular alkalinization increased the magnitude of the rise of [Ca2+]i and force produced by KCl. 5. An intracellular acidification was also associated with contraction in the portal vein and ureter. 6. We conclude that the sarcolemmal Ca(2+)-ATPase produces a significant intracellular acidification while removing Ca2+. Both the acidification and decrease of [Ca2+]i will promote relaxation. Since Ca2+ and protons have opposite effects on many cellula...Continue Reading

References

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Citations

May 18, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ariana Hemara-WahanuiMarion A Maw
May 5, 1999·The Journal of Physiology·C H LeemR D Vaughan-Jones
Jul 27, 1999·The Journal of Physiology·J Larcombe-McDouallS Wray
Mar 17, 2009·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Karen NobleSusan Wray
Jun 22, 2005·Experimental Eye Research·David Krizaj
Jul 13, 2007·Cell Calcium·Rachel Floyd, Susan Wray
Oct 7, 2017·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Ebbe Boedtkjer
Aug 22, 2012·Journal of Vascular Research·Ebbe Boedtkjer, Christian Aalkjaer
Aug 12, 2016·Physiology·Ebbe BoedtkjerChristian Aalkjaer
Mar 3, 2007·Annals of the New York Academy of Sciences·Anatoly ShmygolSteven Thornton
Jul 13, 2004·Molecular Pharmacology·Susan C HernandezKenneth J Kellar
Nov 13, 2002·American Journal of Physiology. Heart and Circulatory Physiology·R D SmithSusan Wray
Feb 8, 2003·Archives of Biochemistry and Biophysics·E A LukyanetzP G Kostyuk
Jul 5, 2008·Journal of Molecular and Cellular Cardiology·Brian Oloizia, Richard J Paul

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