The role of the specificity-determining loop of the integrin beta subunit I-like domain in autonomous expression, association with the alpha subunit, and ligand binding

Biochemistry
J TakagiT A Springer

Abstract

Integrin beta subunits contain a highly conserved I-like domain that is known to be important for ligand binding. Unlike integrin I domains, the I-like domain requires integrin alpha and beta subunit association for optimal folding. Pactolus is a novel gene product that is highly homologous to integrin beta subunits but lacks associating alpha subunits [Chen, Y., Garrison, S., Weis, J. J., and Weis, J. H. (1998) J. Biol. Chem. 273, 8711-8718] and a approximately 30 amino acid segment corresponding to the specificity-determining loop (SDL) in the I-like domain. We find that the SDL is responsible for the defects in integrin beta subunit expression and folding in the absence of alpha subunits. When transfected in the absence of alpha subunits into cells, extracellular domains of mutant beta subunits lacking SDL, but not wild-type beta subunits, were well secreted and contained immunoreactive I-like domains. The purified recombinant soluble beta1 subunit with the SDL deletion showed an elongated shape in electron microscopy, consistent with its structure in alphabeta complexes. The SDL segment is not required for formation of alpha5beta1, alpha4beta1, alphaVbeta3, and alpha6beta4 heterodimers, but is essential for fomation of alph...Continue Reading

Citations

Nov 26, 2010·Proceedings of the National Academy of Sciences of the United States of America·YouDong PanJianFeng Chen
Jul 21, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Koichi YukiRoderic G Eckenhoff
Oct 11, 2005·Annual Review of Cell and Developmental Biology·M A ArnaoutJ-P Xiong
Jun 22, 2006·BMC Cell Biology·A Paul MouldMartin J Humphries
May 10, 2008·BMC Evolutionary Biology·Brent A KnackDavid J Miller
Nov 11, 2014·Nature Structural & Molecular Biology·Xianchi DongTimothy A Springer
Jul 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Andrea ArtoniBarry S Coller
Sep 18, 2002·Immunological Reviews·Junichi Takagi, Timothy A Springer
Jul 13, 2007·Immunological Reviews·Ana Kasirer-FriedeSanford J Shattil
May 25, 2007·Proteins·Mehmet Sen, Glen B Legge
Jul 28, 2007·Virology·Deng-Feng LiXun Xu
Mar 25, 2017·Protein Science : a Publication of the Protein Society·Lingyun WangYuhua Song
Sep 27, 2003·The Journal of Biological Chemistry·Mossaad Abdel-GhanyBendicht U Pauli
Jul 19, 2018·Blood·Dongwen ZhouJieqing Zhu
Sep 14, 2018·Proceedings of the National Academy of Sciences of the United States of America·Aye Myat Myat ThinnJieqing Zhu
Apr 2, 2019·Nature Communications·Swechha M PokharelSantanu Bose
Jan 1, 2014·Current Protocols in Cell Biology·Daiji KiyozumiKiyotoshi Sekiguchi
Oct 1, 2019·Journal of Thrombosis and Haemostasis : JTH·Benoit GuilletAlan T Nurden
Jan 24, 2020·Arteriosclerosis, Thrombosis, and Vascular Biology·Dragana NešićBarry S Coller
Oct 16, 2004·The Journal of Biological Chemistry·Dmitry A SolovjovEdward F Plow
Jan 16, 2020·Life Science Alliance·Daiji KiyozumiKiyotoshi Sekiguchi
Nov 21, 2018·Life Science Alliance·Daiji KiyozumiKiyotoshi Sekiguchi
Sep 10, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Louise Carstensen GjelstrupThomas Vorup-Jensen
Dec 11, 2013·The Journal of Biological Chemistry·Xian-Wei WangJin-Xing Wang
Aug 17, 2018·Biology Open·Catherine G GalbraithJames A Galbraith
Aug 6, 2004·Journal of Medicinal Chemistry·Luciana MarinelliHorst Kessler

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