The role of the T7 Gp2 inhibitor of host RNA polymerase in phage development.

Journal of Molecular Biology
Dhruti SavaliaKonstantin Severinov

Abstract

Bacteriophage T7 relies on its own RNA polymerase (RNAp) to transcribe its middle and late genes. Early genes, which include the viral RNAp gene, are transcribed by the host RNAp from three closely spaced strong promoters-A1, A2, and A3. One middle T7 gene product, gp2, is a strong inhibitor of the host RNAp. Gp2 is essential and is required late in infection, during phage DNA packaging. Here, we explore the role of gp2 in controlling host RNAp transcription during T7 infection. We demonstrate that in the absence of gp2, early viral transcripts continue to accumulate throughout the infection. Decreasing transcription from early promoter A3 is sufficient to make gp2 dispensable for phage infection. Gp2 also becomes dispensable when an antiterminating element boxA, located downstream of early promoters, is deleted. The results thus suggest that antiterminated transcription by host RNAp from the A3 promoter is interfering with phage development and that the only essential role for gp2 is to prevent this transcription.

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Citations

Nov 26, 2013·Proceedings of the National Academy of Sciences of the United States of America·Lucia B Rothman-Denes
Nov 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Brian BaeSeth A Darst
Feb 15, 2011·Journal of Molecular Biology·Carol SheppardSiva R Wigneshweraraj
Nov 7, 2012·Virology·Evgeny KlimukKonstantin Severinov
Oct 4, 2011·Journal of Molecular Biology·Vladimir MeklerKonstantin Severinov
Apr 6, 2013·RNA Biology·Carol SheppardSivaramesh Wigneshweraraj
Apr 5, 2014·Bacteriophage·Bing LiuSivaramesh Wigneshweraraj
Jul 24, 2012·Molecular Cell·Ellen JamesSivaramesh Wigneshweraraj
Nov 25, 2016·Nature Communications·Carol SheppardFinn Werner
Oct 5, 2017·Nucleic Acids Research·Paramita SarkarSivaramesh Wigneshweraraj
Jun 27, 2014·Journal of Virology·Pieter-Jan CeyssensRob Lavigne
May 10, 2017·Nucleic Acids Research·Aline Tabib-SalazarSivaramesh Wigneshweraraj
May 24, 2018·Proceedings of the National Academy of Sciences of the United States of America·Aline Tabib-SalazarSivaramesh Wigneshweraraj
Dec 5, 2018·Scientific Reports·Christian Møller-OlsenAntonia P Sagona
Feb 2, 2021·Microbial Cell Factories·Patrick StargardtJuergen Mairhofer
Apr 24, 2020·ACS Synthetic Biology·Patrick StargardtJuergen Mairhofer

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