The role of the unique motifs in the amino-terminal region of PKN on its enzymatic activity

Biochemical and Biophysical Research Communications
M KitagawaY Ono

Abstract

The yeast two-hybrid system and in vitro binding assay were carried out to characterize the interaction between the amino-terminal and carboxyl-terminal region of PKN. It was revealed that the amino-terminal region containing the regulatory domain associated with the carboxyl-terminal catalytic region. A synthetic peptide, corresponding to the amino acid residues of PKN from 39 to 53, with substitution of isoleucine46 with serine was shown to become a potent substrate for PKN, and its wild type synthetic peptide inhibited the phosphorylation by PKN. These results suggest that the amino-terminal region of PKN contains the pseudosubstrate sequence and acts as an autoinhibitory region.

Citations

May 19, 2000·The Biochemical Journal·A L Bishop, A Hall
Oct 27, 2010·Molecular and Cellular Biology·Sean W WallaceAlan Hall
Jul 14, 2011·The Biochemical Journal·Alejandra CollazosPeter J Parker
Dec 3, 2014·Biochimica Et Biophysica Acta·J Martin-LiberalM Linch
Sep 11, 2014·Nature Communications·Nicolas StranskyChristoph Lengauer
Mar 22, 2001·The Journal of Biological Chemistry·K OishiY Ono
Jan 23, 2010·Nature Reviews. Molecular Cell Biology·Carine RossePeter J Parker
Sep 18, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yimin ZhuWei Duan
Feb 28, 1998·The Journal of Biological Chemistry·P FlynnP J Parker
Nov 17, 2007·The Journal of Biological Chemistry·Rakhee ModhaHelen R Mott
Jun 6, 2020·Biomolecular NMR Assignments·Georgios SophocleousHelen R Mott
Feb 2, 2021·Biochemical Society Transactions·Georgios SophocleousHelen R Mott
May 29, 1997·Biochemical and Biophysical Research Communications·K MatsuzawaM Inagaki
Jan 21, 2000·Brain Research. Molecular Brain Research·H ShibataY Ono
Jul 31, 2021·Developmental Biology·Adrian Garcia-Concejo, Dan Larhammar
Mar 1, 2011·Biochemistry·Catherine L HutchinsonDarerca Owen

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