The role of TNF-α in regulating ketamine-induced hippocampal neurotoxicity

Archives of Medical Science : AMS
Xiaozhu ZhengYanfei Xia

Abstract

Ketamine is commonly used in pediatric anesthesia but recent studies have shown that it could induce neurotoxicity in the developing brain. The inflammatory cytokine, tumor necrosis factor α (TNF-α) is involved in the pathogenesis of various types of neurodegenerations. In the present study, we examined whether TNF-α may regulate ketamine-induced neurotoxicity in the hippocampus of neonatal mouse. The in vitro organotypic culture of hippocampal slices was used to investigate the gain-of-function and loss-of-function effect of TNF-α modulation on ketamine-induced hippocampal neurotoxicity. Also, western blotting analysis was used to examine the relative pathways associated with TNF-α modulation. In the in vivo Morris water maze test, TNF-α was genetically silenced to see if memory function was improved after anesthesia-induced memory impairment. In in vitro experiments, adding TNF-α enhanced (112.99 ±5.4%, p = 0.015), whereas knocking down TNF-α ameliorated (46.8 ±11.6%, p = 0.003) ketamine-induced apoptosis in hippocampal CA1 neurons in the organotypic culture. Western blotting showed that addition of TNF-α reduced (67.1 ±3.7%, p = 0.022), whereas downregulation of TNF-α increased (126.87 ±8.5%, p = 0.004) the phosphorylation o...Continue Reading

Citations

Oct 26, 2016·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Bo ChenHongliang Liu
Jun 1, 2017·Current Opinion in Anaesthesiology·Federico BilottaShaun E Gruenbaum
Jan 29, 2019·Early Intervention in Psychiatry·Oliver D RobertsonSeetal Dodd
Nov 7, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Stefania SchiavoneMaria Grazia Morgese
Aug 18, 2020·Brain Research Bulletin·Fan YangHongtao Liu

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BETA
transfection
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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis