The role of toll-like and protease-activated receptors in the expression of cytokines by gingival fibroblasts stimulated with the periodontal pathogen Porphyromonas gingivalis

Cytokine
Eleonor PalmHazem Khalaf

Abstract

Porphyromonas gingivalis is a periodontitis-associated pathogen and interactions between the bacterium and gingival fibroblasts play an important role in development and progression of periodontitis, an inflammatory disease leading to degeneration of tooth-supporting structures. Gingival fibroblasts, which expresses protease activated receptors (PARs) as well as toll-like receptors (TLRs), produces inflammatory mediators upon bacterial challenges. In this study, we elucidated the importance of PAR1, PAR2, TLR2 and TLR4 for the expression and secretion of CXCL8, interleukin-6 (IL-6), transforming growth factor-β1 (TGF-β1) and secretory leukocyte inhibitor (SLPI). Human gingival fibroblasts were transfected with small-interfering RNA against the target genes, and then stimulated with P. gingivalis wild-type W50 and W50-derived double rgp mutant E8 and kgp mutant K1A. TLR2-silencing reduced P. gingivalis-induced CXCL8 and IL-6. IL-6 was also reduced after PAR1-silencing. No effects were observed for TGF-β1. SLPI was suppressed by P. gingivalis and silencing of PAR1 as well as TLR2, gave additional suppression at the mRNA level. TLR4 was not involved in the regulation of the investigated mediators. CXCL8 and IL-6 are important for ...Continue Reading

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Citations

Feb 16, 2016·Journal of Dentistry·Esteban Rodriguez HerreroWim Teughels
May 12, 2017·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Kartheyaene JayaprakashTorbjörn Bengtsson
Jan 26, 2017·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Eleonor PalmHazem Khalaf
Jul 21, 2017·Journal of Clinical Periodontology·Joerg MeyleMark Herzberg
Jun 23, 2020·Mediators of Inflammation·Hong-Yan GuoRen-Yong Jia
Dec 4, 2019·Molecular Medicine Reports·Shuxun ShiGuiwen Yang
Feb 13, 2020·Brazilian Oral Research·João Antonio Chaves de SouzaPedro Paulo Chaves de Souza

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