The role of toll-like receptors in the protective effect of melatonin against doxorubicin-induced pancreatic beta cell toxicity

Life Sciences
Eylem TaskinYusuf Sevgiler

Abstract

Doxorubicin, an anticancer drug, has a toxic effect on many tissues such as heart, pancreas, liver, kidney, and testis. The aim of current study is to investigate whether melatonin would be protective in doxorubicin-induced beta (β) cell toxicity via HMGB1/TLR2/TLR4/MAPK/NF-қB signaling pathway. Human pancreatic β cell (1.1B4) was used in the present study. Four experimental groups were created as control, melatonin (10 μM), doxorubicin (2 μM) and the combination of melatonin with doxorubicin. Following 24-h treatment, Mitogen-activated protein kinase (MAPKs), Toll like receptors (TLRs) including TLR2 and TLR4, pro-and anti-apoptotic protein expression levels were determined by western blotting. Total antioxidant (TAS), oxidant status (TOS) and oxidative stress index (OSI) of the cells as well as superoxide dismutase (SOD) levels were determined. Active caspase-8 activity was measured and TUNEL staining was performed to study apoptotic pathways. Mitochondrial membrane potential (MMP), some protein expressions and F-actin distribution were analyzed. Doxorubicin caused to depolarize MMP, resulting in enhancing apoptosis by activation of caspase-8 via MAPKs/NF-кB pathway via elevation of TOS and decreasing TAS. Also, doxorubicin d...Continue Reading

References

Jul 20, 2007·Immunology and Cell Biology·Ashish Banerjee, Steve Gerondakis
Jul 24, 2008·Journal of Cancer Survivorship : Research and Practice·Charles J StavaRena Vassilopoulou-Sellin
Jun 11, 2010·Essays in Biochemistry·Melissa J Parsons, Douglas R Green
Feb 12, 2011·Cell Death and Differentiation·D V KryskoP Vandenabeele
Feb 1, 2012·Journal of Pineal Research·Abdulhadi C UguzJosé A Pariente
Jun 11, 2014·World Journal of Gastroenterology : WJG·Juan Vaz, Roland Andersson
Jul 26, 2014·Molecular Medicine Reports·Sameer E Al-HarthiAbdel-Moneim M Osman
Oct 14, 2014·Frontiers in Immunology·Takumi Kawasaki, Taro Kawai
Oct 16, 2015·Journal of Pineal Research·Frederic NduhirabandiSandrine Lecour
Feb 11, 2016·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Celal GuvenHandan Akcakaya
May 31, 2016·Journal of Cachexia, Sarcopenia and Muscle·Edson Alves de Lima JuniorJose Cesar Rosa Neto
Jun 4, 2016·Toxicological Sciences : an Official Journal of the Society of Toxicology·Emma A HeartJoshua P Gray
May 11, 2018·Oxidative Medicine and Cellular Longevity·Stefania GoriniElisabetta Ferraro
Aug 18, 2018·Expert Opinion on Drug Metabolism & Toxicology·Hamed Haghi-AminjanMohammad Abdollahi
Sep 21, 2018·American Society of Clinical Oncology Educational Book·Saro Armenian, Smita Bhatia
Sep 22, 2018·Journal of Cellular Physiology·Bagher FarhoodAmirhossein Sahebkar
Sep 25, 2018·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Mira A LankiCaj H Haglund
May 22, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nancy S Younis, Maged E Mohamed
Jun 1, 2019·Acta Endocrinologica : the International Journal of the Romanian Society of Endocrinology·M A R HadjzadehZ Keshavarzi
Jun 5, 2019·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Nadeem AkhterSardar Sindhu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis