PMID: 9432016Jan 31, 1998Paper

The role of transforming growth factor-beta in PEG-rHuMGDF-induced reversible myelofibrosis in rats

British Journal of Haematology
M YanagidaT Tokiwa

Abstract

Pegylated recombinant human megakaryocyte growth and development factor (PEG-rHuMGDF) injected at a suprapharmacologic dose (100 microg/kg) daily for 5 d in normal rats caused marked increases in marrow megakaryocytes and platelet counts at 6-8 d followed by gradual decreases to control levels at 10-20 d. Interestingly, in addition to the expected thrombopoiesis, PEG-rHuMGDF was associated with myelofibrosis with a predominance of reticulin fibres at day 10 followed by complete normalization by day 20. At 6-8 d, the levels of transforming growth factor-beta1 (TGF-beta1) in the extracellular fluid of the marrow, the platelet poor plasma, and the platelet extract were increased 23-, 7- and 2-fold, respectively. The elevated levels of TGF-beta1 were gradually reduced to baseline levels at 13-20 d in accordance with the normalization of myelofibrosis and thrombopoiesis. An ultrastructural analysis showed that large fragments of megakaryocytes were deposited in the marrow parenchyma of PEG-rHuMGDF-treated rats at day 6. PEG-rHuMGDF administration at pharmacologic doses (1 and 10 microg/kg) did not induce the deposition of reticulin fibres in the marrow. These findings suggest that TGF-beta1 leaked from megakaryocytes is involved in ...Continue Reading

Citations

May 25, 2001·International Journal of Hematology·A Tefferi
Oct 23, 2013·Current Hematologic Malignancy Reports·Niccolò BartalucciAlessandro M Vannucchi
Sep 5, 2001·Leukemia Research·D P SteensmaA Tefferi
Jan 20, 2000·Baillière's Clinical Haematology·J T Reilly
Apr 27, 2000·The New England Journal of Medicine·A Tefferi
Oct 20, 2006·The New England Journal of Medicine·James B BusselJanet L Nichol
May 12, 2006·The New England Journal of Medicine·Kenneth Kaushansky
Sep 1, 1999·Current Opinion in Hematology·E M Cramer
Nov 21, 2007·Hematology·Biree AndemariamJames B Bussel
Feb 10, 2007·Blood·David J Kuter
Sep 1, 2004·Internal Medicine·David DingliAyalew Tefferi
Nov 5, 2008·Internal Medicine·Kumiko MatsuiToshiaki Kamei
Jan 13, 2010·Saudi Journal of Gastroenterology : Official Journal of the Saudi Gastroenterology Association·Fazal A DanishSaeeda Yasmin
May 1, 2007·Annals of the New York Academy of Sciences·Anna Rita MigliaccioFrancesco A Manzoli
Oct 4, 2007·British Journal of Haematology·David J KuterRobert P Hasserjian
Sep 10, 2011·British Journal of Haematology·Waleed GhanimaJames B Bussel
Mar 31, 1999·Baillière's Clinical Haematology·A E von dem BorneM de Haas
Jan 13, 2015·Cellular and Molecular Life Sciences : CMLS·Alessandro MalaraAlessandra Balduini
Nov 19, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Ayalew Tefferi
Feb 9, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Ayalew TefferiAnimesh Pardanani
Jun 26, 2004·Pathologie-biologie·Alessandro M VannucchiAnna Rita Migliaccio
Jun 17, 2005·Annals of the New York Academy of Sciences·Anna Rita MigliaccioFrancesco A Manzoli
Dec 22, 2007·Expert Review of Cardiovascular Therapy·Ara Metjian, Charles S Abrams
Oct 24, 2002·Blood·Hédia ChagraouiFrançoise Wendling
Aug 1, 2002·Blood·Alessandro Maria VannucchiAnna Rita Migliaccio
May 30, 2003·The Oncologist·Ayalew Tefferi
Feb 9, 2019·The Journal of Experimental Medicine·Alessandro MalaraAlessandra Balduini
Feb 25, 2009·Clinical Pharmacology and Therapeutics·M Liem-MoolenaarJ Burggraaf
Nov 24, 1999·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·G Barosi
Jan 23, 2021·Biomolecules·Mariarita SpampinatoGiuseppe A Palumbo

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