The role of transforming growth factor-beta1, -beta2, and -beta3 in androgen-responsive growth of NRP-152 rat prostatic epithelial cells

Journal of Cellular Physiology
M S LuciaD Danielpour

Abstract

We have investigated the role of autocrine/paracrine TGF-beta secretion in the regulation of cell growth by androgens as demonstrated by its inhibition by two androgen response modifiers; the nonsteroidal antiandrogen hydroxyflutamide (OHF), believed to act by inhibiting androgen binding to androgen receptors, or finasteride, an inhibitor of 5alpha-reductase, the enzyme necessary for the conversion of testosterone to 5alpha-dihydrotestosterone (DHT), using the nontumorigenic rat prostatic epithelial cell line NRP-152. Growth of these cells was stimulated three- to sixfold over control by either testosterone or DHT under serum-free culture conditions. This was accompanied by a two- to threefold decrease in the secretion rate of TGF-beta1, -beta2, and -beta3. Finasteride reversed the ability of testosterone but not DHT to stimulate growth and downregulate expression of TGF-beta1, -beta2, and -beta3 in a dose-dependent fashion, suggesting that this activity of testosterone required its conversion to DHT. OHF antagonized the stimulatory effects of DHT on NRP-152 cell growth but could reverse the inhibitory effects of DHT only on TGF-beta2 and TGF-beta3 and not TGF-beta1 secretion. This suggests that either TGF-beta1 regulation by D...Continue Reading

References

Dec 1, 1990·The Journal of Clinical Endocrinology and Metabolism·J Geller
Jan 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·A L SchuurmansE Mulder
Jan 1, 1990·Annual Review of Cell Biology·J Massagué
Jan 1, 1990·Annals of the New York Academy of Sciences·D A MillerH L Moses
Mar 1, 1989·Molecular and Cellular Endocrinology·G WildingE Gelmann
Aug 1, 1987·The Journal of Cell Biology·L M WakefieldM B Sporn
Apr 1, 1994·Baillière's Clinical Endocrinology and Metabolism·V A Randall
Dec 1, 1993·Journal of Clinical Pharmacology·D P McDonnellM A Gleeson
Dec 1, 1993·The Journal of Steroid Biochemistry and Molecular Biology·C KnabbeK D Voigt
Jan 14, 1993·Journal of Immunological Methods·D Danielpour

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Citations

Apr 6, 2005·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·David Danielpour
Feb 19, 2003·The Journal of Steroid Biochemistry and Molecular Biology·M BlanchèreI Mowszowicz
Apr 10, 2009·Carcinogenesis·Cheryl L ThompsonLi Li
May 3, 2002·The Journal of Biological Chemistry·Jerry E ChipukDavid Danielpour
Dec 20, 2015·PloS One·Jennifer S MyersQing-Xiang Amy Sang
Nov 15, 2001·The Journal of Biological Chemistry·Jerry E ChipukDavid Danielpour
Aug 2, 2003·Oncogene·Steven A HayesRaymond C Bergan
Oct 17, 2018·Breast Cancer Research : BCR·Elin BorgenBjørn Naume
Sep 3, 2011·International Journal of Urology : Official Journal of the Japanese Urological Association·Hyung Jong NamNam Cheol Park
May 6, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jieping HuTing Sun
Jan 21, 2016·The Journal of Biological Chemistry·Eswar ShankarDavid Danielpour

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