The role of transmembrane electrochemical potential and phosphorylation of PS II proteins in temperature induced light emission from ATP-treated lettuce thylakoids

Photosynthesis Research
J Farineau


Changes in characteristics of flash-induced thermoluminescence (TL) glow curves in thylakoids of lettuce following incubation of the organelles with ATP, under illumination or in the dark, were investigated. TL bands were induced by 1 or 2 flashes fired at -10°C or 1°C in thylakoids: TL curves in control thylakoids which were dark-adapted or submitted to an illumination without ATP, can be deconvoluted as the sum of one single B band and minor contributions. In thylakoids incubated for 90 s with 0.5 mM ATP, either under light or in the dark (after a 90 s preillumination), bands presented complex shapes; after deconvolution, they appeared composed of a B band with a low Ea (activation energy): 0.6 e.v. as compared to 0.75 in control, and a supplementary band peaking at about 10°C. The band at low temperature was suppressed by low concentrations (10-20 nM) of valinomycin, nigericin or FCCP as well as by 10 mM ammonium chloride, leaving B bands with the same characteristics as in control material. Finally with higher nigericin concentrations, the bands became single B bands with high Ea (0.9 e.v.). These characteristics would define 3 different energized states (in the form of a transmembrane electrochemical potential) for thylako...Continue Reading


Jun 5, 1979·Biochimica Et Biophysica Acta·U Schreiber, M Avron
Jun 5, 1979·Biochimica Et Biophysica Acta·M Avron, U Schreiber
Oct 15, 1984·The Biochemical Journal·Z G Cerović, M Plesnicar
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A W RutherfordY Inoue

Related Concepts

Adenosine Triphosphate
Alkaline Phosphatase
Ammonium Chloride
Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone
Thylakoid polypeptides

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.