The role of triiodothyronine hormone and mechanically-stressed endothelial cell paracrine signalling synergism in gene reprogramming during hBMSC-stimulated osteogenic phenotype in vitro

Molecular and Cellular Endocrinology
Rodrigo A da SilvaWillian F Zambuzzi

Abstract

We therefore investigated whether there is synergism between triiodothyronine (T3) hormone and trophic molecules released from mechanically-stressed endothelial cells (EC-enriched medium) in osteogenic phenotype by mapping classical repertory of genes. Although there are studies reporting the efficiency of T3 hormone on bone cells, it is scarce considering their effect in conjunction with other physiologically active molecules, such as those released by the active endothelial cells. To address this issue, human bone marrow-derived mesenchymal stem cells (hBMSCs) were treated with EC-enriched medium subjected to shear-stress up to 72 h in vitro, in conjunction or not with T3 hormone. Although our results found an important synergism considering these parameters on modulating key bone-related gene markers, such as on the alkaline phosphatase (ALP) behavior (at both mRNA and protein content), contributing for osteoblast differentiation, important genes such as OSTERIX and RUNX2 were significantly down-expressed, while a over-expression of RANKL was found when the conjunction effect of T3 and endothelial paracrine signaling was considered. In addition, T3 hormone over expressed both OCT4 and NANOG genes in a DNA epigenetic-independ...Continue Reading

Citations

Mar 23, 2021·Journal of Materials Science. Materials in Medicine·Célio da Costa FernandesGisele Faria
Jul 27, 2021·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·N PanahiB Larijani
Mar 29, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zizhen SiYang Hui
Sep 4, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Célio Junior da Costa FernandesWillian Fernando Zambuzzi

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