The role of tumor necrosis factor-alpha in apoptosis of dorsal root ganglion cells induced by herniated nucleus pulposus in rats

Spine
Yasuaki MurataKjell Olmarker

Abstract

The mechanisms of apoptosis underlying a characteristic tissue reaction at the surface of the dorsal root ganglion (DRG) exposed to nucleus pulposus were studied in rats with special reference to the role of tumor necrosis factor-alpha (TNF). To study the characteristic tissue reaction at the surface of the DRG exposed to nucleus pulposus with special reference to the role of TNF. Nucleus pulposus cells are capable of producing TNF. Recently, local application of nucleus pulposus was shown to induce a characteristic tissue reaction at the DRG surface due to apoptosis. Recombinant TNF was applied to the DRG to mimic L4-L5 disc herniation in rats. The DRGs were resected 24 hours after surgery. Sections of the specimens were processed for immunohistochemistry using antisera to single-stranded DNA, Caspase 3, and TNF, and observed by light and electron microscopy. Typical apoptotic changes of the cell nuclei were observed in the DRG after application of TNF. The presence of single-stranded DNA, Caspase 3, and TNF further confirmed the occurrence of DRG cell apoptosis. TNF seemed to play a key role in induction of apoptosis of DRG cells, which resembled that induced by application of nucleus pulposus.

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Citations

Oct 30, 2013·Nature Reviews. Rheumatology·Makarand V Risbud, Irving M Shapiro
Mar 5, 2015·Cell Proliferation·Zheng LiWilliam Ka Kei Wu
Sep 13, 2018·Journal of Cellular and Molecular Medicine·Kaori SuyamaMasahiko Watanabe
Oct 23, 2019·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Ryohei SatoShin-Ichi Konno
Sep 1, 2010·Revista Brasileira De Ortopedia·André Luiz de Souza GravaHelton Luiz Aparecido Defino
Nov 21, 2019·Molecular Medicine Reports·Chang LiuYue Zhou

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis