PMID: 9435273Jan 22, 1998Paper

The role of yeast VDAC genes on the permeability of the mitochondrial outer membrane

The Journal of Membrane Biology
A C LeeM Colombini

Abstract

In addition to the POR1 gene, which encodes the well-characterized voltage dependent anion-selective channel (YVDAC1) of the mitochondrial outer membrane, the yeast Saccharomyces cerevisiae contains a second gene (POR2) encoding a protein (YVDAC2) with 50% sequence identity to YVDAC1. Mitochondria isolated from yeast cells deleted for the POR1 gene (delta por1) had a profoundly reduced outer membrane permeability as measured by the ability of an intermembrane space dehydrogenase to oxidize exogenously added NADH. Mitochondria missing either YVDAC1 or both YVDAC1 and YVDAC2 showed a 2-fold increase in the rate of NADH oxidation when the outer membrane was deliberately damaged. Mitochondria from parental cells showed only a 10% increase indicating that the outer membrane is highly permeable to NADH. In the absence of YVDAC1, we calculate that the outer membrane permeability to NADH is reduced 20-fold. The low NADH permeability in the presence of YVDAC2 was not due to the low levels of YVDAC2 expression as mitochondria from cells expressing levels of YVDAC2 comparable to those of YVDAC1 in parental cells showed no substantial increase in NADH permeability, indicating a minimal role of YVDAC2 in this permeability. The residual perm...Continue Reading

Citations

Jul 9, 2009·Plant Cell Reports·Tomonobu KusanoYoshihiro Takahashi
Jan 27, 2010·Apoptosis : an International Journal on Programmed Cell Death·Vidyaramanan GanesanMarco Colombini
Sep 17, 2005·Journal of Bioenergetics and Biomembranes·Malgorzata WojtkowskaHanna Kmita
Jul 26, 2008·Journal of Bioenergetics and Biomembranes·C A Stein, Marco Colombini
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