Jun 2, 2020

The role of ZFP580 in the regulation of rat VSMCs migration by ATRA and its mechanism

Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology
Guo-Dong WuWen-Cheng Zhang

Abstract

To investigate the probable roles of the novel C2H2 zinc finger transcription factor ZFP580 on all-transretinoic acid (ATRA)-regulated VSMCs migration and underlying mechanisms. Rat aortic VSMCs were isolated, cultured and identified. VSMCs were treated with ATRA at the concentrations of 0, 5, 10 or 20 μmol/L for 24 hours. The migration ability of VSMCs was observed in each group and compared with control group which was treated by 0 μmol/L ATRA. The mRNA and protein expression levels of ZFP580 were detected by QPCR and Western blot. ZFP580 protein expression in VSMCs was detected under ATRA stimulation when ERK inhibitor PD98059 was used to inhibit the protein expression of ERK. Adenovirus transfection technology was used to obtain VSMCs with overexpression or low expression of ZFP580, and QPCR and Western blot were used to detect the mRNA and protein levels of MMP-2, MMP-9 and ZFP580. On the 10th day of VSMCs culture, immunofluorescence showed that SM22 alpha antibody, as a specific marker of smooth muscle cells, was positive. Compared to the control group, VSMCs migration was reduced by 32%, 43%, and 59% in the group of 5, 10, and 20 μmol/L ATRA pretreatment. Compared with the control group, VSMCs treated by 20 μmol/L ATRA r...Continue Reading

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Mentioned in this Paper

Signal Pathways
Protein Expression
Quantitative Real-Time Polymerase Chain Reaction
Aorta
Stimulation Procedure
Detected (Finding)
Immunofluorescence Assay
ZFP580 protein, rat
MMP2
Matrix Metalloproteinase 9

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