The roles of connective tissue growth factor in the development of anastomotic esophageal strictures

Archives of Medical Science : AMS
Haibin ZhaoYaoyi Wu

Abstract

The aim of this study was to investigate the roles of connective tissue growth factor (CTGF) in the development of anastomotic strictures after surgical repair of the esophagus. Tissues collected from the patients were divided into three groups based on the results of endoscopy and clinical grading. Patients without dysphagia after esophagectomy were used as the control population. The protein levels of CTGF, TGF-β1, Smad2, and Smad4 were determined by immunohistochemistry (IHC) and western blot analyses, while the mRNA levels of the two growth factors were evaluated by real-time polymerase chain reaction. Compared with the control group, significantly increased (p < 0.01) levels of CTGF and TGF-β1 protein were observed in the anastomotic stenosis (AS) group, and levels of the two proteins detected by the IHC and western blot analyses were also significantly increased with the increasing severity of stenosis (p < 0.05). The mRNA levels of CTGF and TGF-β1 in the tissues collected from the patients with stenosis were significantly up-regulated (p < 0.05) as compared with those from the control group. In addition, the levels of Smad2 and Smad4 protein were also significantly increased (p < 0.05) with the increasing severity of ste...Continue Reading

Citations

Aug 11, 2021·Colorectal Disease : the Official Journal of the Association of Coloproctology of Great Britain and Ireland·Rachael Elizabeth CliffordDale Vimalachandran

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Methods Mentioned

BETA
Fluorescence
Assay
biopsy
PCR
electrophoresis

Software Mentioned

VIDAS
UVPro
SPSS

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