The roles of different porcine cytochrome P450 enzymes and cytochrome b5A in skatole metabolism

Animal : an International Journal of Animal Bioscience
P WiercinskaE James Squires

Abstract

Boar taint is the unfavourable odour and taste from pork fat, which results in part from the accumulation of skatole (3-methylindole, 3MI). The key enzymes in skatole metabolism are thought to be cytochrome P450 2E1 (CYP2E1) and cytochrome 2A (CYP2A); however, the cytochrome P450 (CYP450) isoform responsible for the production of the metabolite 6-hydroxy-3-methylindole (6-OH-3MI, 6-hydroxyskatole), which is thought to be involved in the clearance of skatole, has not been established conclusively. The aim of this study was to characterize the role of porcine CYP450s in skatole metabolism by expressing them individually in the human embryonic kidney HEK293-FT cell line. This system eliminates the problems of the lack of specificity of antibodies, inhibitors and substrates for CYP450 isoforms in the pig, and contributions of any other CYP450s that would be present. The results show that pig CYP1A1, CYP2A19, CYP2C33v4, CYP2C49, CYP2E1 and CYP3A and human CYP2E1 (hCYP2E1) are all capable of producing the major skatole metabolite 3-methyloxyindole (3MOI), as well as indole-3-carbinol (I3C), 5-hydroxy-3-methylindole (5-OH-3MI), 6-OH-3MI, 2-aminoacetophenone (2AAP) and 3-hydroxy-3-methyloxindole. CYP2A19 produced the highest amount of ...Continue Reading

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Citations

Aug 4, 2012·Proceedings. Biological Sciences·Jérôme MichelettaAntje Engelhardt
Apr 10, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Bernard Thierry
Jan 26, 2016·Journal of Agricultural and Food Chemistry·Christoph GerlachMatthias Wuest
Sep 18, 2014·Journal of Veterinary Pharmacology and Therapeutics·S X Hu
Nov 20, 2014·Computational and Structural Biotechnology Journal·Martin Krøyer Rasmussen, Galia Zamaratskaia
Oct 4, 2012·The Journal of Steroid Biochemistry and Molecular Biology·Matthew A Gray, E James Squires
Dec 4, 2014·Animal : an International Journal of Animal Bioscience·F Borrisser-PairóG Zamaratskaia
Mar 10, 2016·Asian-Australasian Journal of Animal Sciences·Sheng QingkaiYing Wu
Apr 6, 2016·Toxicologic Pathology·Kristi L HelkeCharlotte Hollinger
Sep 2, 2018·Basic & Clinical Pharmacology & Toxicology·Viktoriia BurkinaGalia Zamaratskaia
Apr 16, 2016·Animal : an International Journal of Animal Bioscience·C BruniusG Zamaratskaia
Feb 6, 2017·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Stefan F C VaessenEvita van de Steeg
Nov 2, 2019·Animals : an Open Access Journal From MDPI·Michel Bonneau, Ulrike Weiler
Dec 28, 2019·Animals : an Open Access Journal From MDPI·Kevin KressVolker Stefanski
Sep 20, 2020·Animals : an Open Access Journal From MDPI·E James SquiresJocelyn Cameron
Mar 17, 2020·Archives of Toxicology·Martin Krøyer Rasmussen

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