PMID: 2107533Feb 25, 1990Paper

The roles of indoleglycerol phosphate and the TrpI protein in the expression of trpBA from Pseudomonas aeruginosa

Nucleic Acids Research
M Chang, I P Crawford

Abstract

The TrpI protein belongs to the LysR-family of procaryotic regulatory proteins. Members of this family share a characteristic similarity of their N-terminal amino acid sequences, and many of them are activators of divergently transcribed genes or operons. In Pseudomonas aeruginosa, the genes for tryptophan synthase, trpBA, are regulated by indoleglycerol phosphate (InGP) and TrpI. We demonstrate here that in the absence of InGP, the binding site of TrpI is located in the -52 to -77 region of the trpBA promoter; in the presence of InGP, the binding region is extended to the -32 region. In addition, two major, slow moving protein-DNA complexes are seen in gel retardation assays: the faster moving complex is formed in the absence of InGP and the amount of the slower moving complex is greatly enhanced in the presence of InGP. These results suggest that the binding of a second TrpI protein molecule, promoted by InGP, plays a crucial role in activating the expression of the trpBA gene pair.

References

Mar 1, 1978·FEBS Letters·F Sanger, A R Coulson
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M F ChristmanB N Ames
Apr 1, 1989·Biochimie·I P Crawford, L Eberly
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T D Tullius, B A Dombroski
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S HenikoffJ C Wallace
Oct 24, 1986·Nucleic Acids Research·J L Paluh, C Yanofsky
Jan 1, 1986·Annual Review of Microbiology·B W Holloway, A F Morgan
Nov 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·S N CohenR B Helling
Mar 1, 1973·Molecular & General Genetics : MGG·D H CalhounR A Jensen
May 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·R F Baker, C Yanofsky
Aug 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·I P Crawford, I C Gunsalus
Jan 11, 1984·Nucleic Acids Research·L M LagriminiJ E Donelson
Aug 20, 1963·Biochimica Et Biophysica Acta·H SAITO, K I MIURA

❮ Previous
Next ❯

Citations

Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·K GoethalsM Holsters
Jul 21, 2006·Journal of Bacteriology·Melicent C PeckSharon R Long
Sep 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Gary XieRoy A Jensen
Apr 1, 2008·Current Opinion in Microbiology·Enrique MerinoCharles Yanofsky
Dec 1, 2006·Journal of Molecular Biology·Silvia PicossiAbraham L Sonenshein
Aug 1, 1993·Molecular Microbiology·S LindquistS Normark
Nov 11, 2009·Acta Biochimica Et Biophysica Sinica·Bihe HouGuofan Hong
Jun 11, 1998·Journal of Bacteriology·R RayaA Chopin
May 8, 2021·Frontiers in Microbiology·Nanami SakataYasuhiro Ishiga
Aug 1, 1992·Journal of Bacteriology·G N GussinA Ishihama
Nov 1, 1994·Journal of Bacteriology·A F Lamblin, J A Fuchs
Sep 1, 1997·Journal of Bacteriology·S PiñeiroG N Gussin
Jun 1, 1991·Journal of Bacteriology·C Y HanC S Harwood
Jun 1, 1991·Journal of Bacteriology·J G Gao, G N Gussin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.