Abstract
Micronuclei (MNi) are extensively used to evaluate genotoxic effects and chromosome instability. However, the roles of kinetochore of MN in mitosis have not been completely addressed. The HeLa CENP B-GFP H2B-mCherry cells are applied to address these questions via the long-term live-cell imaging. In the cells, the kinetochore-positive micronucleus (K+MN) contained CENP B-GFP, while the kinetochore-negative micronucleus (K-MN) did not. K-MN-bearing cells produced much more chromosome fragments than did MN-free cells. Most of the chromosome fragments eventually merged into K-MNi. K+MN-bearing cells yielded more kinetochore-positive lagging chromosomes (K+LCs) and K+MNi than MN-free cells did. The results suggested the differences in the fates of K+MNi and K-MNi in mitosis. The cycle of K-MN → Chromosome fragment → K-MN may occur in generations of K-MN-bearing cells, while part of K+MNi might reincorporate into the main nucleus. The K+MN-bearing cells prolonged significantly duration of mitosis compared with MN-free cells. The presence of micronuclei, regardless of K-MN and K+MN, enhanced apoptosis cell death. And K+MN-bearing cells were inclined to apoptosis more than K-MN-bearing cells. The results suggested differences in fates...Continue Reading
References
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·M J Martinez-ExpositoP K Sorger
Sep 10, 2003·Mutagenesis·Gui-Rong DingJunji Miyakoshi
Oct 11, 2005·Trends in Cell Biology·Gordon K ChanTim J Yen
Jun 17, 2006·Carcinogenesis·Marta MattiuzzoFrancesca Degrassi
Oct 11, 2008·Mutation Research·Xiaotang RaoQinghua Shi
Apr 14, 2010·PloS One·Koh-ichi UtaniNoriaki Shimizu
Aug 10, 2010·Mutation Research·Manabu YasuiMasamitsu Honma
Apr 14, 2011·Chemosphere·Laila BenameurAlain Botta
May 6, 2011·DNA Repair·Yun HuangQinghua Shi
Jan 20, 2012·Nature·Karen CrastaDavid Pellman
Sep 25, 2012·Mutation Research·Minh C VuongDavid A Eastmond
May 29, 2015·Nature·Cheng-Zhong ZhangDavid Pellman
Oct 8, 2015·Mutagenesis·David J TweatsDean B Evans
Mar 5, 2016·Mutation Research·Erkang Jiang
May 21, 2016·Human & Experimental Toxicology·L da Cunha Menezes SouzaJrc Meireles
May 26, 2016·Asian Pacific Journal of Cancer Prevention : APJCP·Shikha TewariAnoop K Srivastav
Jun 18, 2016·Oxidative Medicine and Cellular Longevity·Nicole SchuppAugust Heidland
Jul 5, 2016·Chromosoma·Harsh Nagpal, Tatsuo Fukagawa
Jul 13, 2016·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Shambhu RoyMarilyn J Aardema
Aug 20, 2016·Archives of Toxicology·Mariona TerradasAnna Genescà