PMID: 2125211Dec 15, 1990Paper

The roles of phospholipase D and a GTP-binding protein in guanosine 5'-[gamma-thio]triphosphate-stimulated hydrolysis of phosphatidylcholine in rat liver plasma membranes

The Biochemical Journal
K M HurstG J Barritt

Abstract

1. Guanosine 5'-[gamma-thio]triphosphate (GTP[S]) stimulated by 50% the rate of release of [3H]choline and [3H]phosphorylcholine in rat liver plasma membranes labelled with [3H]choline. About 70% of the radioactivity released in the presence of GTP[S] was [3H]choline and 30% was [3H]phosphorylcholine. 2. The hydrolysis of phosphorylcholine to choline and the conversion of choline to phosphorylcholine did not contribute to the formation of [3H]choline and [3H]phosphorylcholine respectively. 3. The release of [3H]choline from membranes was inhibited by low concentrations of SDS or Triton X-100. Considerably higher concentrations of the detergents were required to inhibit the release of [3H]phosphorylcholine. 4. Guanosine 5'-[beta gamma-imido]triphosphate and guanosine 5'-[alpha beta-methylene]triphosphate, but not adenosine 5'-[gamma-thio]-triphosphate, stimulated [3H]choline release to the same extent as did GTP[S]. The GTP[S]-stimulated [3H]choline release was inhibited by guanosine 5'-[beta-thio]diphosphate, GDP and GTP but not by GMP. 5. It is concluded that, in rat liver plasma membranes, (a) GTP[S]-stimulated hydrolysis of phosphatidylcholine is catalysed predominantly by phospholipase D with some contribution from phosphol...Continue Reading

Citations

Jan 17, 1994·FEBS Letters·J N Kanfer, D McCartney
Jan 1, 1996·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·M G Houle, S Bourgoin
Jun 13, 2012·Hippocampus·Timothy GoodmanPeter McCaffery
Dec 13, 2019·Progress in Lipid Research·M I McDermottV A Bankaitis
Mar 1, 1994·The American Journal of Physiology·I Guillemain, B Rossignol
May 24, 1996·Chemistry and Physics of Lipids·S Cockcroft
Aug 11, 1994·Biochimica Et Biophysica Acta·C BenistantR Rubin
May 8, 1993·Biochimica Et Biophysica Acta·Z GatalicaJ B Hoek
Apr 14, 1994·Biochimica Et Biophysica Acta·J H Exton
Jun 1, 1994·Molecular and Cellular Biology·H JiangD A Foster

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