The roles of RGD and grooved topography in the adhesion, morphology, and differentiation of C2C12 skeletal myoblasts

Biotechnology and Bioengineering
Peng-Yuan WangWei-Bor Tsai

Abstract

Both chemical and topographic cues are crucial for the development of skeletal muscle. In this study, the relative roles of both signals in regard to cell adhesion, morphology, and differentiation of C2C12 skeletal myoblasts were investigated. Grooved polystyrene substrates containing grooves with approximately 900 nm in width with 600 nm ridge spans and 665 nm in depth were conjugated with the cell adhesion peptide arginine-glycine-aspartic acid (RGD). RGD conjugation significantly enhanced the adhesion, growth and differentiation of C2C12 cells. On the other hand, anisotropic topography primarily directed the direction and alignment of myoblasts and myotubes. The results in this study provide information regarding the relative roles of chemical and topographic cues in musculoskeletal myogenesis, and are of interest to applications in muscle tissue engineering.

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Citations

Aug 21, 2012·Journal of Materials Science. Materials in Medicine·Peng-Yuan WangWei-Bor Tsai
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