The roles of the catalytic and noncatalytic activities of Rpd3L and Rpd3S in the regulation of gene transcription in yeast

PloS One
Daniella Yeheskely-HayonY Kassir

Abstract

In budding yeasts, the histone deacetylase Rpd3 resides in two different complexes called Rpd3L (large) and Rpd3S (small) that exert opposing effects on the transcription of meiosis-specific genes. By introducing mutations that disrupt the integrity and function of either Rpd3L or Rpd3S, we show here that Rpd3 function is determined by its association with either of these complexes. Specifically, the catalytic activity of Rpd3S activates the transcription of the two major positive regulators of meiosis, IME1 and IME2, under all growth conditions and activates the transcription of NDT80 only during vegetative growth. In contrast, the effects of Rpd3L depends on nutrients; it represses or activates transcription in the presence or absence of a nitrogen source, respectively. Further, we show that transcriptional activation does not correlate with histone H4 deacetylation, suggesting an effect on a nonhistone protein. Comparison of rpd3-null and catalytic-site point mutants revealed an inhibitory activity that is independent of either the catalytic activity of Rpd3 or the integrity of Rpd3L and Rpd3S.

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Citations

Dec 3, 2015·The Journal of Microbiology·Jueun KimJung-Shin Lee
Apr 28, 2018·FEMS Yeast Research·Yona Kassir
Aug 31, 2018·G3 : Genes - Genomes - Genetics·Kwan Yin LeeMarc D Meneghini
Oct 11, 2020·Current Genetics·Safia Mahabub SautyKrassimir Yankulov
Mar 14, 2021·The Journal of Biological Chemistry·Sophie L CookeRobertus A M de Bruin

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Methods Mentioned

BETA
acetylation
immunoprecipitation
PCR
ChIP
FACS
histone acetylation

Software Mentioned

WinMDI

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