The roles of the zinc finger transcription factors XlnR, ClrA and ClrB in the breakdown of lignocellulose by Aspergillus niger

AMB Express
Roxane RauloDavid B Archer

Abstract

Genes encoding the key transcription factors (TF) XlnR, ClrA and ClrB were deleted from Aspergillus niger and the resulting strains were assessed for growth on glucose and wheat straw, transcription of genes encoding glycosyl hydrolases and saccharification activity. Growth of all mutant strains, based in straw on measurement of pH and assay of glucosamine, was impaired in relation to the wild-type (WT) strain although deletion of clrA had less effect than deletion of xlnR or clrB. Release of sugars from wheat straw was also lowered when culture filtrates from TF deletion strains were compared with WT culture filtrates. Transcript levels of cbhA, eglC and xynA were measured in all strains in glucose and wheat straw media in batch culture with and without pH control. Transcript levels from cbhA and eglC were lowered in all mutant strains compared to WT although the impact of deleting clrA was not pronounced with expression of eglC and had no effect on xynA. The impact on transcription was not related to changes in pH. In addition to impaired growth on wheat straw, the ΔxlnR strain was sensitive to oxidative stress and displayed cell wall defects in the glucose condition suggesting additional roles for XlnR. The characterisation ...Continue Reading

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Citations

Apr 21, 2017·Frontiers in Microbiology·Ariann E Mendoza-MartínezJesús Aguirre
Jan 4, 2018·International Journal of Molecular Sciences·Muhammad AdnanGuodong Lu
Nov 25, 2017·BMC Genomics·Birgit S GrubenRonald P De Vries
Mar 7, 2019·Nature Reviews. Cancer·Ming-Ru WuTimothy K Lu
Jun 16, 2017·Biotechnology for Biofuels·Tiziano BenocciRonald P de Vries
Oct 4, 2018·Frontiers in Bioengineering and Biotechnology·Glaucia Emy Okida MidorikawaRobert Neil Gerard Miller
Sep 29, 2017·Scientific Reports·Joanna E KowalczykRonald P de Vries
Aug 1, 2020·Frontiers in Bioengineering and Biotechnology·Teng ZhangChun Li
Oct 17, 2020·Biotechnology for Biofuels·Raphael GabrielSteven W Singer
Sep 2, 2021·Journal of Applied Microbiology·Chengqiang XiaXin Song

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Methods Mentioned

BETA
PCR
ELISA
co-immunoprecipitation
pull down

Software Mentioned

GXCapture
ImageJ

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