The ROS-generating oxidase Nox1 is required for epithelial restitution following colitis

Experimental Animals
Masayoshi KatoTohru Kamata

Abstract

Accumulating evidence suggests that reactive oxygen species (ROS) generated by endogenous metabolic enzymes are involved in a variety of intracellular mechanisms. In particular, superoxide-generating NADPH oxidase (Nox) 1 is highly expressed in the colon and has been implicated in physiological and pathophysiological states of colon tissues. However, its role in tissue repair following colitis has not been fully elucidated. Our study using experimental colitis in mice showed that repair of the mucosal layer did not occur in Nox1-deficient mice following dextran sulfate sodium-induced colitis. This was accompanied by inhibition of proliferation, cell survival, migration, and terminal differentiation (generation of goblet cells) of crypt progenitor cells, as determined by histochemical analyses. Furthermore, Nox1 expression as well as ROS production in the colon crypt was increased during the repair process, and Nox1 deficiency suppressed these events. The results suggest that Nox1 promotes colon mucosal wound repair by sustaining the bioactivity of crypt progenitor cells and plays a crucial role in the epithelial restitution in the case of damage associated with colitis.

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Citations

Dec 22, 2016·Journal of Clinical Medicine·Odile WéraCécile Oury
Aug 8, 2018·Wiener medizinische Wochenschrift·Michael BreitenbachJiri Hasek
May 11, 2018·Mucosal Immunology·Gabriella Aviello, Ulla G Knaus
Jun 6, 2018·Frontiers in Immunology·Franziska MollKatrin Schröder
Dec 29, 2016·World Journal of Gastroenterology : WJG·Fong-Fong ChuBinghui Shen
May 31, 2018·Nature Reviews. Gastroenterology & Hepatology·Eva RathDirk Haller
Feb 6, 2019·Proceedings of the National Academy of Sciences of the United States of America·Nikolai HeckerMichael Hiller
Aug 9, 2019·Cardiovascular Research·Fabian HahnerKatrin Schröder
Jan 13, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yasuka MatsunagaJay K Kolls
Dec 15, 2020·Oxidative Medicine and Cellular Longevity·Hanna Grauers WiktorinAnna Martner
Jun 22, 2021·Frontiers in Immunology·Alexia Dumas, Ulla G Knaus

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Methods Mentioned

BETA
confocal microscopy

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