Abstract
Cardiac stem cells (CSCs)-based therapy provides a promising avenue for the management of ischemic heart diseases. However, engrafted CSCs are subjected to acute cell apoptosis in the ischemic microenvironment. Here, stem cell antigen 1 positive (Sca-1(+)) CSCs proved to own therapy potential were cultured and treated with H2O2 to mimic the ischemia situation. As autophagy inhibitor, 3-methyladenine (3MA), inhibited H2O2-induced CSCs apoptosis, thus we demonstrated that H2O2 induced autophagy-dependent apoptosis in CSCs, and continued to find key proteins responsible for the crosstalk between autophagy and apoptosis. Nuclear Receptor Subfamily 4 Group A Member 2 (NR4A2), increased upon cardiomyocyte injury with unknown functions in CSCs, was increased by H2O2. NR4A2 siRNA attenuated H2O2 induced autophagy and apoptosis in CSCs, which suggested an important role of NR4A2 in CSCs survival in ischemia conditions. Reactive oxygen species (ROS) and NF-κB (P65) subunit were both increased by H2O2. Either the ROS scavenger, N-acetyl-l-cysteine (NAC) or NF-κB signaling inhibitor, bay11-7082 could attenuate H2O2-induced autophagy and apoptosis in CSCs, which suggested they were involved in this process. Furthermore, NAC inhibited NF-κB ...Continue Reading
References
Mar 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alice N McEvoyEvelyn P Murphy
Jun 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Konrad UrbanekPiero Anversa
Jul 16, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Yue Zhao, Dennis Bruemmer
Nov 16, 2010·Nature Cell Biology·Olga SirinMargaret A Goodell
Jan 14, 2011·European Heart Journal·Zhaokang ChengMark A Sussman
Apr 13, 2011·Stem Cells and Development·Marijke W MaijenburgCarlijn Voermans
Apr 10, 2012·CNS & Neurological Disorders Drug Targets·Zhen ZhangWeidong Le
May 26, 2012·Development·Chang-Hwan ParkSang-Hun Lee
Aug 18, 2012·Molecular Neurobiology·Elzbieta JandaVincenzo Mollace
Nov 6, 2012·Cell Biology International Reports·Leilei LuJingjing Lu
Mar 15, 2013·Autophagy·Jun-Lin GuanJue Zhang
Jun 29, 2013·Methods in Molecular Biology·Toshio NagaiIssei Komuro
Jan 23, 2014·Stem Cells·Xuan OuHal E Broxmeyer
Aug 12, 2014·BMC Biotechnology·Hao WangZhiwei Xu
Dec 23, 2014·Lancet·UNKNOWN GBD 2013 Mortality and Causes of Death Collaborators
Jan 17, 2015·The International Journal of Biochemistry & Cell Biology·Ji Hoon YuHyeyoung Kim
Mar 11, 2015·The Journal of Biological Chemistry·Takeshi WatanabeHidenori Ichijo
Mar 20, 2015·Stem Cells·Yubo TangMaik Stiehler
Apr 15, 2015·Journal of Cardiovascular Pharmacology and Therapeutics·Ling HuangWei Zhou
Apr 18, 2015·BMC Cancer·Yang CaoHua Yan
Jul 15, 2015·Cell Death and Differentiation·Z LiX Yang
Oct 21, 2015·Frontiers in Cell and Developmental Biology·Amanda Finan, Sylvain Richard
Feb 24, 2016·Scientific Reports·Dong LiangFeng Cao
Apr 14, 2016·Biochimica Et Biophysica Acta·Wenjing LiJing Zhao
Jul 21, 2016·Molecular and Cellular Biochemistry·Mohammad Jodeiri FarshbafMohammad Hossein Nasr-Esfahani
Dec 25, 2016·Cancer Letters·Kai SunLixin Wei
Jan 4, 2017·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Shuo ZhangYu Liu
Jan 9, 2017·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Zhi-Jing HeKai Wang
Jan 10, 2017·Journal of Cellular Physiology·Shuling SongQiang Zhang
Citations
Sep 22, 2019·Scientific Reports·Tomoya TakedaTakashi Nakamura
Oct 2, 2019·International Journal of Molecular Sciences·Gwang-Bum ImSuk Ho Bhang
Jan 11, 2020·Bioscience Reports·Yuqing ZouWan Li
Apr 18, 2020·Frontiers in Pharmacology·Yinghui LiYingdai Gao
Dec 10, 2020·Cellular & Molecular Biology Letters·Liqun ChenYiyi Zhao
Aug 7, 2021·Stem Cell Research & Therapy·Chan WuGang Li
Nov 6, 2018·Journal of Agricultural and Food Chemistry·Ming SunGuang Yang