PMID: 6404908May 1, 1983Paper

The rough endoplasmic reticulum and the Golgi apparatus visualized using specific antibodies in normal and tumoral prolactin cells in culture

The Journal of Cell Biology
C TougardA Tixier-Vidal

Abstract

Antibodies directed against membrane components of dog pancreas rough endoplasmic reticulum (A-RER) and rat liver Golgi apparatus (A-Golgi) (Louvard, D., H. Reggio, and G. Warren, 1982, J. Cell Biol. 92:92-107) have been applied to cultured rat prolactin (PRL) cells, either normal cells in primary cultures, or clonal GH3 cells. In normal PRL cells, the A-RER stained the membranes of the perinuclear cisternae as well as those of many parallel RER cisternae. The A-Golgi stained part of the Golgi membranes. In the stacks it stained the medial saccules and, with a decreasing intensity, the saccules of the trans side, as well as, in some cells, a linear cisterna in the center of the Golgi zone. It also stained the membrane of many small vesicles as well as that of lysosomelike structures in all cells. In contrast, it never stained the secretory granule membrane, except at the level of very few segregating granules on the trans face of the Golgi zone. In GH3 cells the A-RER stained the membrane of the perinuclear cisternae, as well as that of short discontinuous flat cisternae. The A-Golgi stained the same components of the Golgi zone as in normal PRL cells. In some cells of both types the A-Golgi also stained discontinuous patches o...Continue Reading

References

Dec 1, 1978·The Journal of Cell Biology·A TartakoffM Détraz
Sep 1, 1977·The Histochemical Journal·A B Novikoff, P M Novikoff
Oct 1, 1967·The Journal of Cell Biology·B R BrinkleyL C Richardson
Apr 1, 1966·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R C Graham, M J Karnovsky
Dec 1, 1981·The Journal of Cell Biology·M G Farquhar, G E Palade
Jan 1, 1982·The Journal of Cell Biology·D LouvardG Warren

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Citations

Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M C Williams
Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·P VolpeJ Meldolesi
Jun 1, 1984·The Journal of Cell Biology·Z D SmithJ D Jamieson
Jan 1, 1992·The European Journal of Neuroscience·Emilio ClementiJacopo Meldolesi
Nov 1, 1987·Journal of Neurochemistry·J O GonatasD Louvard
Sep 1, 1993·Trends in Cell Biology·A Luini, M A De Matteis

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