The rpoH gene encoding heat shock sigma factor sigma32 of psychrophilic bacterium Colwellia maris

Extremophiles : Life Under Extreme Conditions
Seiji YamauchiHidenori Hayashi

Abstract

The rpoH gene encoding a heat shock sigma factor, sigma(32), was cloned from the psychrophilic bacterium Colwellia maris. The deduced amino acid sequence of sigma(32) from C. maris is more than 60% homologous to that of sigma(32) from mesophilic bacteria. The RpoH box, a 9-amino-acid sequence region (QRKLFFNLR) specific to sigma(32), and two downstream box sequences complementary to a part of 16S rRNA were identified. Primer extension analysis showed that the C. maris rpoH is expressed from only one sigma(70)-type promoter. Northern blot analysis showed that the level of rpoH mRNA was clearly increased at 20 degrees C, a temperature that induces heat shock in this organism. In the presence of an inhibitor of transcriptional initiation, the degradation of rpoH mRNA was much slower at 20 degrees C than at 10 degrees C. Thus, increased stability of the rpoH mRNA might be responsible for the rpoH mRNA accumulation. The predicted secondary structure of the 5'-region of C. maris rpoH mRNA was different from the conserved patterns reported for most mesophilic bacteria, and the base pairing of the downstream boxes appeared to be less stable than that of Escherichia coli rpoH mRNA. Thus, essential features that ensure the HSP expression...Continue Reading

References

Jun 1, 1992·Journal of Bacteriology·M LonettoC A Gross
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·H NagaiT Yura
Aug 1, 1989·Journal of Bacteriology·Q P Wang, J M Kaguni
Aug 1, 1993·Molecular Microbiology·Bernd Bukau
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·H NagaiT Yura
Apr 1, 1996·Journal of Bacteriology·A ReisenauerL Shapiro
Apr 15, 1996·FEMS Microbiology Letters·R Segal, E Z Ron
Nov 26, 1998·International Journal of Systematic Bacteriology·I YumotoH Okuyama
May 14, 1999·Current Opinion in Microbiology·T Yura, K Nakahigashi
May 3, 2000·International Journal of Food Microbiology·F ArsèneBernd Bukau
Jan 5, 2001·Nucleic Acids Research·J Ramírez-SantosM C Gómez-Eichelmann
Dec 1, 2001·Nucleic Acids Research·A NockerF Narberhaus
Dec 20, 2002·Angewandte Chemie·Stefan Walter, Johannes Buchner
Sep 3, 2003·Molecular Microbiology·Regine Hengge, Bernd Bukau
Apr 16, 2004·Extremophiles : Life Under Extreme Conditions·Seiji YamauchiHidenori Hayashi

Citations

Sep 22, 2012·Extremophiles : Life Under Extreme Conditions·Seiji YamauchiHidenori Hayashi

Related Concepts

RpoH protein, E coli
Heat shock proteins
RNA Conformation
Early Promoters, Genetic
RNA, Bacterial
Poly(A) Tail
RNA Polymerase Sigma Factor H
Gene Expression Regulation, Bacterial
Homologous Sequences, Amino Acid
Gammaproteobacteria

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.