The S2 Subunit of Infectious Bronchitis Virus Beaudette Is a Determinant of Cellular Tropism

Journal of Virology
Erica BickertonPaul Britton

Abstract

The spike (S) glycoprotein of the avian gammacoronavirus infectious bronchitis virus (IBV) is comprised of two subunits (S1 and S2), has a role in virulence in vivo, and is responsible for cellular tropism in vitro We have previously demonstrated that replacement of the S glycoprotein ectodomain from the avirulent Beaudette strain of IBV with the corresponding region from the virulent M41-CK strain resulted in a recombinant virus, BeauR-M41(S), with the in vitro cell tropism of M41-CK. The IBV Beaudette strain is able to replicate in both primary chick kidney cells and Vero cells, whereas the IBV M41-CK strain replicates in primary cells only. In order to investigate the region of the IBV S responsible for growth in Vero cells, we generated a series of recombinant IBVs expressing chimeric S glycoproteins, consisting of regions from the Beaudette and M41-CK S gene sequences, within the genomic background of Beaudette. The S2, but not the S1, subunit of the Beaudette S was found to confer the ability to grow in Vero cells. Various combinations of Beaudette-specific amino acids were introduced into the S2 subunit of M41 to determine the minimum requirement to confer tropism for growth in Vero cells. The ability of IBV to grow and ...Continue Reading

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Citations

Mar 5, 2019·The Journal of Veterinary Medical Science·Atsushi KatoKazumoto Shibuya
Aug 24, 2019·Veterinary World·Zafar Ahmed BhuiyanZahed Uddin Mahmood Khan
Nov 12, 2020·Animals : an Open Access Journal From MDPI·Miguel Guzmán, Héctor Hidalgo
Jan 13, 2021·The Journal of Veterinary Medical Science·Masaji MaseHiroshi Iseki
Aug 24, 2021·The Journal of General Virology·Phoebe Stevenson-LeggettErica Bickerton

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Datasets Mentioned

BETA
AJ311317
X04722
JN192154
KY933090

Methods Mentioned

BETA
electron microscopy
reverse transcription-PCR
confocal microscopy
PCR

Software Mentioned

Gap4
Leica LAS AF
Staden

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