The Saccharomyces cerevisiae 14-3-3 proteins are required for the G1/S transition, actin cytoskeleton organization and cell wall integrity.

Genetics
Francisca LottersbergerMaria Pia Longhese

Abstract

14-3-3 proteins are highly conserved polypeptides that participate in many biological processes by binding phosphorylated target proteins. The Saccharomyces cerevisiae BMH1 and BMH2 genes, whose concomitant deletion is lethal, encode two functionally redundant 14-3-3 isoforms. To gain insights into the essential function(s) shared by these proteins, we searched for high-dosage suppressors of the growth defects of temperature-sensitive bmh mutants. Both the protein kinase C1 (Pkc1) and its upstream regulators Wsc2 and Mid2 were found to act as high dosage suppressors of bmh mutants' temperature sensitivity, indicating a functional interaction between 14-3-3 and Pkc1. Consistent with a role of 14-3-3 proteins in Pkc1-dependent cellular processes, shift to the restrictive temperature of bmh mutants severely impaired initiation of DNA replication, polarization of the actin cytoskeleton, and budding, as well as cell wall integrity. Because Pkc1 acts in concert with the Swi4-Swi6 (SBF) transcriptional activator to control all these processes, the defective G(1)/S transition of bmh mutants might be linked to impaired SBF activity. Indeed, the levels of the G(1) cyclin CLN2 transcripts, which are positively regulated by SBF, were drama...Continue Reading

References

Mar 1, 1992·The Journal of Cell Biology·D E Levin, E Bartlett-Heubusch
Apr 1, 1995·Current Opinion in Cell Biology·D E Levin, B Errede
Oct 13, 1994·Nature·W J FantlL T Williams
Jul 1, 1994·Molecular and Cellular Biology·F R CrossA H Tinkelenberg
Mar 1, 1993·The Journal of Cell Biology·D J Lew, S I Reed
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·D GelperinS Lemmon
Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·M Tyers
Dec 6, 1996·Science·K Nasmyth
Oct 6, 1997·Genes & Development·L Van Aelst, C D'Souza-Schorey
Dec 31, 1997·Genes & Development·J L BrownM Peter
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·J VernaR Ballester
Aug 28, 1998·Molecular Microbiology·N MarcouxD Pallotta
May 18, 1999·Molecular and Cellular Biology·M RajavelD E Levin
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·F Winston, P Sudarsanam
Oct 6, 1999·The Journal of Cell Biology·P A Delley, M N Hall
Apr 19, 2000·The Biochemical Journal·P C Van Der HoevenW J Van Blitterswijk

❮ Previous
Next ❯

Citations

Sep 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Aaron BoudreauMina J Bissell
Oct 28, 2011·Genome Génome / Conseil National De Recherches Canada·Emily L Humphrey-DixonRobin Lock
Dec 18, 2009·Science in China. Series C, Life Sciences·Rong ZhaoQi Jin
Jul 1, 2008·Fungal Genetics and Biology : FG & B·Natalia Mielnichuk, José Pérez-Martín
Dec 7, 2007·Trends in Biochemical Sciences·Maria Zannis-HadjopoulosMario Callejo
Jul 20, 2010·Yeast·Rosaura Rodicio, Jürgen J Heinisch
Mar 5, 2017·European Journal of Cell Biology·Ravinder Kumar
Jan 23, 2019·World Journal of Microbiology & Biotechnology·Liang ShiMingwen Zhao
Nov 26, 2009·Journal of Cell Science·Wafaa Yahyaoui, Maria Zannis-Hadjopoulos

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.