The Saccharomyces cerevisiae OXA1 gene is required for the correct assembly of cytochrome c oxidase and oligomycin-sensitive ATP synthase

FEBS Letters
N AltamuraG Dujardin

Abstract

The nuclear gene OXA1 was first isolated in Saccharomyces cerevisiae and found to be required at a post-translational step in cytochrome c oxidase biogenesis, probably at the level of assembly. Mutations in OXA1 lead to a complete respiratory deficiency. The protein Oxa1p is conserved through evolution and a human homolog has been isolated by functional complementation of a yeast oxa1- mutant. In order to further our understanding of the role of Oxa1p, we have constructed two yeast strains in which the OXA1 open reading frame was almost totally deleted. Cytochrome spectra and enzymatic activity measurements show the absence of heme aa3 and of a cytochrome c oxido-reductase activity and dramatic decrease of the oligomycin sensitive ATPase activity. Analysis of the respiratory complexes in non-denaturing gels reveals that Oxa1p is necessary for the correct assembly of the cytochrome c oxidase and the ATP synthase complex.

References

Dec 1, 1978·European Journal of Biochemistry·A PansiniS Papa
Jan 1, 1992·Current Genetics·D C ChenT T Kuo
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S H Ackerman, A Tzagoloff
Jan 1, 1995·Critical Reviews in Biochemistry and Molecular Biology·L A Grivell
Jun 30, 1995·The Journal of Biological Chemistry·D M GlerumA Tzagoloff
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·N BonnefoyG Dujardin

❮ Previous
Next ❯

Citations

Feb 1, 1997·Annals of Neurology·P L AdamsD M Turnbull
Apr 2, 2005·Current Opinion in Microbiology·Martin van der LaanArnold J M Driessen
Nov 5, 1999·Biochimica Et Biophysica Acta·W VoosN Pfanner
Dec 4, 2001·FEMS Microbiology Letters·M R YenM H Saier
Sep 7, 2000·Microbiology and Molecular Biology Reviews : MMBR·H TjalsmaJ M van Dijl
Sep 13, 2005·Annual Review of Microbiology·Mechthild PohlschröderAlex Haddad
May 21, 2013·Future Medicinal Chemistry·Rex A PalmerDavid R Lisgarten
Feb 1, 2011·Annual Review of Biochemistry·Ross E DalbeyAndreas Kuhn
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y Saint-GeorgesG Dujardin
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·K HellR A Stuart
Dec 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Toshiharu SuzukiMasasuke Yoshida
Nov 19, 2005·Proceedings of the National Academy of Sciences of the United States of America·Adnan HasonaL Jeannine Brady
Sep 6, 2011·Biochimica Et Biophysica Acta·Kai Stefan Dimmer, Doron Rapaport
Aug 31, 2010·Biochimica Et Biophysica Acta·Peng Wang, Ross E Dalbey
Apr 30, 1998·Annals of Medicine·H T Jacobs
Feb 11, 2015·Biochimica Et Biophysica Acta·Thilo Rühle, Dario Leister

❮ Previous
Next ❯

Related Concepts

Related Feeds

ATP Synthases

ATP synthases are enzymes located in the inner mitochondrial membrane that catalyze the synthesis of ATP during cellular respiration. Discover the latest research on ATP synthases here.