The Saccharomyces cerevisiae SCRaMbLE system and genome minimization.

Bioengineered Bugs
Jessica S Dymond, Jef D Boeke

Abstract

We have recently reported the first partially synthetic eukaryotic genome. Saccharomyces cerevisiae chromosomes synIXR and semi-synVIL are fully synthetic versions of the right arm of chromosome IX and the telomeric segment of the left arm of chromosome VI, respectively, and represent the beginning of the synthetic yeast genome project, Sc2.0, that progressively replaces native yeast DNA with synthetic sequences. We have designed synthetic chromosome sequences according to principles specifying a wild-type phenotype, highly stable genome, and maintenance of genetic flexibility. Although other synthetic genome projects exist, the Sc2.0 approach is unique in that we have implemented design specifications predicted to generate a wild-type phenotype until induction of "SCRaMbLE," an inducible evolution system that generates significant genetic diversity. Here we further explore the significance of Sc2.0 and show how SCRaMbLE can serve as a genome minimization tool.

References

Mar 11, 1986·Nucleic Acids Research·R H HoessK Abremski
Oct 5, 2001·Nature Reviews. Genetics·Y Yu, A Bradley
Dec 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Hamilton O SmithJ Craig Venter
Sep 6, 2008·Microbiology and Molecular Biology Reviews : MMBR·Elke Nevoigt
Dec 17, 2008·Proceedings of the National Academy of Sciences of the United States of America·Daniel G GibsonClyde A Hutchison
Mar 3, 2010·Nucleic Acids Research·William J BlakeBrian M Baynes
May 22, 2010·Science·Daniel G GibsonJ Craig Venter
Dec 4, 2010·Science·Jay D Keasling

❮ Previous
Next ❯

Citations

Mar 29, 2014·Science·Narayana AnnaluruSrinivasan Chandrasegaran
Jun 27, 2015·FEMS Microbiology Reviews·Daniel Schindler, Torsten Waldminghaus
Apr 14, 2016·Natural Product Reports·Mingzi M ZhangHuimin Zhao
May 10, 2015·Nucleic Acids Research·Leslie A MitchellJef D Boeke
Dec 17, 2014·Frontiers in Bioengineering and Biotechnology·Richard KelwickPaul Freemont
May 5, 2016·Yeast·Damien Coudreuse
Mar 11, 2017·Science·Sarah M RichardsonJoel S Bader
Mar 11, 2017·Science·Guillaume MercyRomain Koszul
Feb 28, 2018·Frontiers in Microbiology·Gazi Sakir HossainMatthew Wook Chang
Nov 23, 2018·Science China. Life Sciences·Zhouqing LuoJunbiao Dai
May 24, 2018·Nature Communications·Lena HochreinBernd Mueller-Roeber
May 24, 2018·Nature Communications·Yi WuJef D Boeke
Jun 23, 2020·Annual Review of Biochemistry·Weimin ZhangJef D Boeke
Mar 12, 2016·The Biochemical Journal·Femi J OlorunnijiW Marshall Stark
Jan 19, 2020·Applied Microbiology and Biotechnology·Lucien AlpersteinVladimir Jiranek
May 1, 2020·Nature Communications·David PerisChris Todd Hittinger
Dec 10, 2020·Biotechnology for Biofuels·Elizabeth L I WightmanHelena K M Nevalainen
Dec 5, 2020·Microorganisms·Elizabeth L I WightmanHelena K M Nevalainen
Jul 22, 2018·Biotechnology Advances·Binbin ChenMatthew Wook Chang
Jul 24, 2021·Chemical Reviews·Yajie WangHuimin Zhao
Oct 18, 2019·ACS Synthetic Biology·Glen-Oliver F GowersZoltan Takáts

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.